From Dysplasia to Carcinoma: Expression Patterns of Dermokine, Matriptase, and Tryptase in OPMD.

IF 2.9 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Oral diseases Pub Date : 2025-07-24 DOI:10.1111/odi.70043

Lara Maria Alencar Ramos Innocentini, Mateus Gonçalves Miranda, Carol Kobori da Fonseca, Pedro Franco Ferreira, Leandro Dorigan de Macedo, Graziela Cavalcanti, Luiz Carlos Conti de Freitas, Ana Carolina Fragoso Motta, Alan Grupioni Lourenço, Katiuchia Uzzun Sales

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引用次数: 0

Abstract

Background: The malignant transformation of oral potentially malignant disorders (OPMDs) lacks reliable molecular markers. Dermokine and matriptase are involved in epithelial differentiation and inflammation, while tryptase is associated with the tumor microenvironment, which may contribute to carcinogenesis. This study investigated these molecules as potential biomarkers for predicting the malignant progression of OPMDs.

Methods: This was a cross-sectional comparative study. Paired normal oral mucosa (NM) and OPMD tissues (n = 21) were assessed, while oral and oropharyngeal squamous cell carcinoma (OOSCC) samples (n = 64) were evaluated using tissue microarrays. Proteomic analysis of five OPMD cases identified dermokine, tryptase, and matriptase as potential biomarkers, further examined through clinical, histopathological, and immunohistochemical analyses across NM, OPMD, and OOSCC tissues.

Results: Females comprised 52% of the OPMD group, whereas males accounted for 87.5% of OOSCC cases. Dermokine, tryptase, and matriptase showed higher expression in OPMD and OOSCC than NM. Dermokine was predominantly observed in OPMD, well-differentiated OOSCC, and NM from patients who progressed to malignancy (p = 0.019). Matriptase expression shifted from membranous in NM to diffuse in OPMD and OOSCC, similarly to tryptase.

Conclusion: Dermokine may represent an early marker of transformation, while altered matriptase patterns could help differentiate dysplasia from carcinoma.

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从发育不良到癌：真皮因子、基质酶和胰蛋白酶在OPMD中的表达模式。

背景：口腔潜在恶性疾病（OPMDs）的恶性转化缺乏可靠的分子标记。真皮因子和基质酶参与上皮分化和炎症，而胰蛋白酶与肿瘤微环境相关，可能参与癌变。本研究探讨了这些分子作为预测OPMDs恶性进展的潜在生物标志物。方法：采用横断面比较研究。对配对的正常口腔黏膜（NM）和OPMD组织（n = 21）进行评估，而对口腔和口咽鳞状细胞癌（OOSCC）样本（n = 64）进行组织微阵列评估。5例OPMD病例的蛋白质组学分析确定了真皮因子、胰蛋白酶和基质酶作为潜在的生物标志物，并通过NM、OPMD和OOSCC组织的临床、组织病理学和免疫组织化学分析进一步检查。结果：女性占OPMD组的52%，男性占OOSCC组的87.5%。真皮因子、胰蛋白酶和基质酶在OPMD和OOSCC中的表达高于NM。Dermokine主要见于OPMD、分化良好的OOSCC和恶性进展的NM患者（p = 0.019）。基质酶的表达从NM的膜性转移到OPMD和OOSCC的弥漫性，类似于胰蛋白酶。结论：真皮因子可能是早期转化的标志，而基质酶模式的改变可以帮助区分非典型增生和癌。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Oral diseases 医学-牙科与口腔外科

CiteScore

7.60

自引率

5.30%

发文量

325

审稿时长

4-8 weeks

期刊介绍： Oral Diseases is a multidisciplinary and international journal with a focus on head and neck disorders, edited by leaders in the field, Professor Giovanni Lodi (Editor-in-Chief, Milan, Italy), Professor Stefano Petti (Deputy Editor, Rome, Italy) and Associate Professor Gulshan Sunavala-Dossabhoy (Deputy Editor, Shreveport, LA, USA). The journal is pre-eminent in oral medicine. Oral Diseases specifically strives to link often-isolated areas of dentistry and medicine through broad-based scholarship that includes well-designed and controlled clinical research, analytical epidemiology, and the translation of basic science in pre-clinical studies. The journal typically publishes articles relevant to many related medical specialties including especially dermatology, gastroenterology, hematology, immunology, infectious diseases, neuropsychiatry, oncology and otolaryngology. The essential requirement is that all submitted research is hypothesis-driven, with significant positive and negative results both welcomed. Equal publication emphasis is placed on etiology, pathogenesis, diagnosis, prevention and treatment.