Genomic epidemiology and antimicrobial resistance of Corynebacterium macclintockiae, the predominant species of human pathogens within the Corynebacterium jeikeium complex.

IF 5.4 2区 医学 Q1 MICROBIOLOGY
Journal of Clinical Microbiology Pub Date : 2025-08-13 Epub Date: 2025-07-24 DOI:10.1128/jcm.00500-25
Sohei Harada, Kohji Komori, Kenya Yukawa, Brian Hayama, Kazumi Takehana, Kageto Yamada, Asako Doi, Tomoo Saga, Masakazu Sasaki, Yoshiro Hadano, Masahiro Suzuki, Kyoko Yokota, Jun Suzuki, Koki Kikuchi, Yohei Doi, Kazuhiro Tateda
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引用次数: 0

Abstract

Genomic characteristics and optimal treatment of Corynebacterium jeikeium remain largely unknown. We collected clinical information and performed whole-genome sequencing analysis of the causative strains of six cases of C. jeikeium infection at a single hospital over a 9-year period. Additionally, whole-genome sequencing analysis was performed on 33 C. jeikeium strains from cases of bloodstream infection at eight hospitals. Antimicrobial susceptibility testing was performed, and the results were compared to the resistance genes identified. Publicly available genome data of strains of C. jeikeium complex, consisting of C. jeikeium sensu stricto, Corynebacterium macclintockiae, and Corynebacterium evansiae, worldwide, were combined with the data from this study to determine the distribution of genomic species. In the single-center study, cases of prosthetic osteoarticular infection, postoperative intra-abdominal infection, and catheter-related bloodstream infection were identified, and the causative strains were genomically identified as C. macclintockiae. All but one isolate (32/33, 97.0%) in the eight-center study identified as C. jeikeium by matrix-assisted laser desorption ionization-time of flight mass spectrometry were also genomically identified as C. macclintockiae. Nosocomial transmission was suggested in three strain pairs by core-genome single nucleotide polymorphism analysis. C. macclintockiae strains were generally multidrug-resistant, but all anti-methicillin-resistant Staphylococcus aureus agents, including teicoplanin, had favorable activity, and the strains without the tet(W) gene (22/38, 57.9%) were susceptible to tetracyclines. Genome analysis of 66 C. jeikeium complex strains collected worldwide, consisting mainly of clinical strains, re-identified 51 strains (77.3%) as C. macclintockiae. This study demonstrates that C. macclintockiae is the major genomic species of the C. jeikeium complex causing human infections.IMPORTANCERecent widespread use of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has facilitated the identification of Corynebacterium spp. in microbiology laboratories, thereby raising awareness of the clinical importance of these organisms. Nevertheless, the accumulation of information on genomic characteristics of Corynebacterium jeikeium has been significantly limited compared to other pathogenic organisms thus far. In this study, we analyzed causative strains of infections identified as C. jeikeium by MALDI-TOF MS, collected from multiple institutions throughout Japan, and found that most of these strains were genomically identified as Corynebacterium macclintockiae, a species that has been newly described recently. Collection of clinical information on selected cases showed that C. macclintockiae indeed caused invasive infections that required intravenous or long-term oral antimicrobial therapy. Additional analyses using genomic data of C. jeikeium complex strains registered in public databases suggest that C. macclintockiae is of global clinical importance.

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杰氏棒状杆菌复合体中人致病菌的优势种——麦克林托基棒状杆菌的基因组流行病学和耐药性。
杰氏棒状杆菌的基因组特征和最佳治疗方法在很大程度上仍然未知。我们收集了一家医院9年间6例耶氏弧菌感染病例的临床资料,并对其致病菌株进行了全基因组测序分析。此外,对来自8家医院血液感染病例的33株大肠杆菌进行了全基因组测序分析。进行药敏试验,并与鉴定的耐药基因进行比较。将世界范围内公开的由严格感大肠弧菌(C. jeeikeium sensu stricto)、macclintockiae、埃文棒状杆菌(Corynebacterium evansiae)组成的大肠弧菌复合体的基因组数据与本研究的数据相结合,确定基因组物种的分布。在单中心研究中,鉴定了假体骨关节感染、术后腹腔内感染和导管相关血流感染病例,基因组鉴定致病菌株为C. macclintockiae。除1株(32/ 33,97.0%)外,其余8个中心的分离物经基质辅助激光解吸电离飞行时间质谱鉴定为C. jekeium,基因组学鉴定为C. macclintockiae。核心基因组单核苷酸多态性分析提示3对菌株存在院内传播。macclintockiae菌株普遍具有多重耐药,但抗甲氧西林耐药的金黄色葡萄球菌药物,包括teicoplanin,都具有良好的活性,没有tet(W)基因的菌株(22/38,57.9%)对四环素敏感。对全球66株以临床菌株为主的洁氏弧菌复合体进行基因组分析,鉴定出51株(77.3%)为macclintockiae。本研究表明,C. macclintockiae是引起人类感染的C. jekeium复合体的主要基因组种。近年来,基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)的广泛应用促进了棒状杆菌在微生物实验室的鉴定,从而提高了对这些生物的临床重要性的认识。然而,迄今为止,与其他致病生物相比,关于杰氏棒状杆菌基因组特征的信息积累明显有限。在这项研究中,我们分析了从日本多个机构收集的MALDI-TOF MS鉴定为C. jeikium的感染病原菌,发现这些菌株大多数被基因组鉴定为macclintockiae,这是一个最近新发现的物种。收集选定病例的临床资料表明,麦克林托克菌确实引起侵袭性感染,需要静脉注射或长期口服抗菌药物治疗。利用公共数据库中登记的C. jekeium复合体菌株的基因组数据进行的进一步分析表明,C. macclintockiae具有全球临床重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Clinical Microbiology
Journal of Clinical Microbiology 医学-微生物学
CiteScore
17.10
自引率
4.30%
发文量
347
审稿时长
3 months
期刊介绍: The Journal of Clinical Microbiology® disseminates the latest research concerning the laboratory diagnosis of human and animal infections, along with the laboratory's role in epidemiology and the management of infectious diseases.
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