Molecular Epidemiology of Hepatitis C Virus Genotypes in Northern Thailand: A Retrospective Study from 2016 to 2024.

IF 2.4 Q2 INFECTIOUS DISEASES
Nang Kham-Kjing, Sirithip Phruekthayanon, Thipsuda Krueyot, Panaddar Phutthakham, Sorasak Intarasoot, Khajornsak Tragoolpua, Kanya Preechasuth, Tanawan Samleerat Carraway, Natedao Kongyai, Woottichai Khamduang
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Abstract

Background: Hepatitis C virus (HCV) remains a significant public health concern in Thailand, with genotype-specific, drug-dependent variations influencing treatment response and disease progression. Despite the availability of pan-genotypic direct-acting antivirals (DAAs), genotype surveillance remains essential for optimizing national elimination strategies. This study thus aims to characterize the molecular distribution of HCV genotypes in northern Thailand. Methods: We conducted a retrospective molecular epidemiological study on 1737 HCV-infected patients who attended the Clinical Microbiology Service Unit (CMSU) Laboratory, Faculty of Associated Medical Sciences, Chiang Mai University between April 2016 and June 2024. HCV genotyping was performed using Sanger sequencing and reverse hybridization line probe assay (LiPA). Results: Genotype 3 was the most prevalent (36.6%), followed by genotype 1 (35.8%) and genotype 6 (27.2%). Subtype 3a (27.2%) predominated, along with 1a (22.1%), 1b (12.6%), and genotype 6 subtypes including 6c to 6l (13.5%) and 6n (6.6%). Males had a higher prevalence of genotype 1, while genotype 3 was more common among females. Temporal analysis revealed a relative increase in genotype 6 prevalence since 2021. Genotype 6 also exhibited significantly higher median viral loads compared to genotypes 1 and 3 (p < 0.0001). Conclusions: This study provides updated evidence on the shifting distribution of HCV genotypes in northern Thailand, particularly the increasing prevalence of genotype 6. These findings underscore the importance of continued molecular surveillance to guide genotype-specific treatment strategies and support Thailand's 2030 HCV elimination goals.

泰国北部丙型肝炎病毒基因型分子流行病学:2016 - 2024年回顾性研究
背景:丙型肝炎病毒(HCV)在泰国仍然是一个重要的公共卫生问题,基因型特异性、药物依赖性变异影响治疗反应和疾病进展。尽管有泛基因型直接作用抗病毒药物(DAAs),但基因型监测对于优化国家消除战略仍然至关重要。因此,本研究旨在表征泰国北部HCV基因型的分子分布。方法:对2016年4月至2024年6月在清迈大学联合医学学院临床微生物服务部(CMSU)实验室就诊的1737例hcv感染患者进行回顾性分子流行病学研究。采用Sanger测序和反向杂交线探针法(LiPA)进行HCV基因分型。结果:以基因3型最多(36.6%),其次为基因1型(35.8%)和基因6型(27.2%)。3a亚型(27.2%)、1a(22.1%)、1b(12.6%)和基因型6亚型包括6c至6l(13.5%)和6n(6.6%)。基因1型男性患病率较高,基因3型女性患病率较高。时间分析显示,自2021年以来基因型6的患病率相对增加。与基因型1和基因型3相比,基因型6也表现出显著更高的中位病毒载量(p < 0.0001)。结论:本研究为泰国北部HCV基因型分布的变化提供了最新证据,特别是基因6型患病率的增加。这些发现强调了持续的分子监测对指导基因型特异性治疗策略和支持泰国2030年消除丙肝病毒目标的重要性。
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来源期刊
Infectious Disease Reports
Infectious Disease Reports INFECTIOUS DISEASES-
CiteScore
5.10
自引率
0.00%
发文量
82
审稿时长
11 weeks
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