Mechanical and Biological Properties of Nondecellularized Human Wharton's Jelly Matrix for Soft Tissue Graft Material: An In Vitro Study.

Q1 Dentistry
Mefina Kuntjoro, Nike Hendrijantini, Eric Priyo Prasetyo, Nurani Atikasari, Made Shintya Danaswari, Guang Hong
{"title":"Mechanical and Biological Properties of Nondecellularized Human Wharton's Jelly Matrix for Soft Tissue Graft Material: An In Vitro Study.","authors":"Mefina Kuntjoro, Nike Hendrijantini, Eric Priyo Prasetyo, Nurani Atikasari, Made Shintya Danaswari, Guang Hong","doi":"10.1055/s-0045-1809981","DOIUrl":null,"url":null,"abstract":"<p><p>This study aims to determine the mechanical properties of hWJM through ultimate tensile strength (UTS) and elastic modulus tests, degradation, and porosity tests. Additionally, it evaluates the biological properties of hWJM by assessing growth factor secretion (fibroblast growth factor-2 [FGF-2] and vascular endothelial growth factor-A [VEGF-A]).For the mechanical tests, two groups were analyzed: group 1 was tested in a wet condition and group 2 in a dry condition. Degradation tests were conducted using phosphate buffer saline (PBS), collagenase enzyme, and simulated body fluid (SBF). The porosity test was conducted using a scanning electron microscope (SEM). For the biological tests, two groups were analyzed: group 1 consisted of nondecellularized hWJM and group 2 of human umbilical cord mesenchymal stem cells. All the data were collected and analyzed statistically.The mean ± standard deviation of the UTS test for group 1 was 0.787 ± 0.356 MPa, while for group 2, it was 1.897 ± 0.582 MPa. The elastic modulus test results were 0.568 ± 0.206 MPa for group 1 and 6.354 ± 2.985 MPa for group 2. The result showed that PBS degradation was 22% on day 14 and 39% on day 28. The collagenase enzyme degraded 15% after 1 hour, 63% after 6 hours, and 74% after 24 hours. SBF degradation rates were 10.3% after 1 week, 10.5% after 2 weeks, 12.5% after 4 weeks, and 13.9% after 8 weeks. The porosity test results showed an average pore size of 66.95 μm. For the biological tests, no significant differences in FGF-2 and VEGF secretion were observed between groups, with the highest secretion in group 1 occurring on day 7.Nondecellularized hWJM has been shown to meet the physical, mechanical, and biological criteria for soft tissue graft in dentistry.</p>","PeriodicalId":12028,"journal":{"name":"European Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"European Journal of Dentistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1055/s-0045-1809981","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Dentistry","Score":null,"Total":0}
引用次数: 0

Abstract

This study aims to determine the mechanical properties of hWJM through ultimate tensile strength (UTS) and elastic modulus tests, degradation, and porosity tests. Additionally, it evaluates the biological properties of hWJM by assessing growth factor secretion (fibroblast growth factor-2 [FGF-2] and vascular endothelial growth factor-A [VEGF-A]).For the mechanical tests, two groups were analyzed: group 1 was tested in a wet condition and group 2 in a dry condition. Degradation tests were conducted using phosphate buffer saline (PBS), collagenase enzyme, and simulated body fluid (SBF). The porosity test was conducted using a scanning electron microscope (SEM). For the biological tests, two groups were analyzed: group 1 consisted of nondecellularized hWJM and group 2 of human umbilical cord mesenchymal stem cells. All the data were collected and analyzed statistically.The mean ± standard deviation of the UTS test for group 1 was 0.787 ± 0.356 MPa, while for group 2, it was 1.897 ± 0.582 MPa. The elastic modulus test results were 0.568 ± 0.206 MPa for group 1 and 6.354 ± 2.985 MPa for group 2. The result showed that PBS degradation was 22% on day 14 and 39% on day 28. The collagenase enzyme degraded 15% after 1 hour, 63% after 6 hours, and 74% after 24 hours. SBF degradation rates were 10.3% after 1 week, 10.5% after 2 weeks, 12.5% after 4 weeks, and 13.9% after 8 weeks. The porosity test results showed an average pore size of 66.95 μm. For the biological tests, no significant differences in FGF-2 and VEGF secretion were observed between groups, with the highest secretion in group 1 occurring on day 7.Nondecellularized hWJM has been shown to meet the physical, mechanical, and biological criteria for soft tissue graft in dentistry.

非脱细胞人类华顿氏果冻基质用于软组织移植材料的力学和生物学特性:体外研究。
本研究旨在通过极限拉伸强度(UTS)和弹性模量测试、降解和孔隙率测试来确定hWJM的力学性能。此外,通过评估生长因子(成纤维细胞生长因子-2 [FGF-2]和血管内皮生长因子- a [VEGF-A])的分泌来评估hWJM的生物学特性。力学试验分为两组:第一组在潮湿条件下进行试验,第二组在干燥条件下进行试验。降解试验采用磷酸缓冲盐水(PBS)、胶原酶和模拟体液(SBF)进行。采用扫描电子显微镜(SEM)进行孔隙率测试。生物学试验分为两组:1组为非脱细胞化hWJM, 2组为人脐带间充质干细胞。收集所有数据并进行统计分析。组1的平均±标准差为0.787±0.356 MPa,组2的平均±标准差为1.897±0.582 MPa。弹性模量试验结果1组为0.568±0.206 MPa, 2组为6.354±2.985 MPa。结果表明,PBS在第14天和第28天的降解率分别为22%和39%。胶原酶在1小时后降解15%,6小时后降解63%,24小时后降解74%。1周后SBF降解率为10.3%,2周后为10.5%,4周后为12.5%,8周后为13.9%。孔隙率测试结果表明,平均孔径为66.95 μm。生物学试验中,各组间FGF-2和VEGF分泌量无显著差异,第1组分泌量在第7天最高。非脱细胞hWJM已被证明满足牙科软组织移植物的物理,机械和生物学标准。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
European Journal of Dentistry
European Journal of Dentistry Dentistry-Dentistry (all)
CiteScore
5.10
自引率
0.00%
发文量
161
期刊介绍: The European Journal of Dentistry is the official journal of the Dental Investigations Society, based in Turkey. It is a double-blinded peer-reviewed, Open Access, multi-disciplinary international journal addressing various aspects of dentistry. The journal''s board consists of eminent investigators in dentistry from across the globe and presents an ideal international composition. The journal encourages its authors to submit original investigations, reviews, and reports addressing various divisions of dentistry including oral pathology, prosthodontics, endodontics, orthodontics etc. It is available both online and in print.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信