Role of Micro RNA Expression as Biomarker in Breast Cancer.

IF 0.7 Q3 MEDICINE, GENERAL & INTERNAL

Annals of African Medicine Pub Date : 2025-07-01 Epub Date: 2025-07-24 DOI:10.4103/aam.aam_78_23

Gitika Nanda Singh, Nijarth Shahi, Parijat Suryavanshi, Krishna Kant Singh, Neetu Nigam

{"title":"Role of Micro RNA Expression as Biomarker in Breast Cancer.","authors":"Gitika Nanda Singh, Nijarth Shahi, Parijat Suryavanshi, Krishna Kant Singh, Neetu Nigam","doi":"10.4103/aam.aam_78_23","DOIUrl":null,"url":null,"abstract":"Background: The most common cancer in women is breast cancer (BC). MicroRNA-21 was one of the first oncomiRs to be found at elevated levels in a number of malignancies, including gliomas, BC, and colorectal cancer (miR-21). MiRNA is associated with processes such as apoptosis, invasion, metastasis, and proliferation, which are known features of cancer. This study aimed to investigate the molecular basis and clinical significance of miR-21 in BC, as microRNAs play a critical role in this disease.Materials and methods: MiR 21 was then analyzed by the quantitative polymerase chain reaction (qPCR) using the Taq-Man probe. The thermal profile included 5 min of denaturation at 95°C, then 40 cycles of denaturation at 95°C for 20 s, annealing at 52°C for 20 s, and extension at 72°C for 30 s. Two separate runs of real-time polymerase chain reaction and reverse transcription (RT) were then performed. Relative quantification of the gene expression levels (fold change) based on computed tomography values was used to examine the data from RT-qPCR for miR-21 and Glyceraldehyde-3-phosphate dehydrogenase (Livak and Schmittgen).Results: The mean fold change in serum miR-21 levels was significantly higher in cases (8.17 ± 2.49) than in controls (1.02 ± 0.23). The mean fold change in serum miR-21 levels in Stages III, IV and lymph nodes were significantly higher than in Stages I and II. The mean fold change in serum miR-21 gene expression was significant with the changes in different Ki67 levels.Conclusions: Our results demonstrate the importance of miR-21 plasma level as a diagnostic and prognostic biomarker for BC.","PeriodicalId":7938,"journal":{"name":"Annals of African Medicine","volume":"24 3","pages":"549-554"},"PeriodicalIF":0.7000,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12380165/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Annals of African Medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4103/aam.aam_78_23","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/7/24 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"MEDICINE, GENERAL & INTERNAL","Score":null,"Total":0}

引用次数: 0

Abstract

Background: The most common cancer in women is breast cancer (BC). MicroRNA-21 was one of the first oncomiRs to be found at elevated levels in a number of malignancies, including gliomas, BC, and colorectal cancer (miR-21). MiRNA is associated with processes such as apoptosis, invasion, metastasis, and proliferation, which are known features of cancer. This study aimed to investigate the molecular basis and clinical significance of miR-21 in BC, as microRNAs play a critical role in this disease.

Materials and methods: MiR 21 was then analyzed by the quantitative polymerase chain reaction (qPCR) using the Taq-Man probe. The thermal profile included 5 min of denaturation at 95°C, then 40 cycles of denaturation at 95°C for 20 s, annealing at 52°C for 20 s, and extension at 72°C for 30 s. Two separate runs of real-time polymerase chain reaction and reverse transcription (RT) were then performed. Relative quantification of the gene expression levels (fold change) based on computed tomography values was used to examine the data from RT-qPCR for miR-21 and Glyceraldehyde-3-phosphate dehydrogenase (Livak and Schmittgen).

Results: The mean fold change in serum miR-21 levels was significantly higher in cases (8.17 ± 2.49) than in controls (1.02 ± 0.23). The mean fold change in serum miR-21 levels in Stages III, IV and lymph nodes were significantly higher than in Stages I and II. The mean fold change in serum miR-21 gene expression was significant with the changes in different Ki67 levels.

Conclusions: Our results demonstrate the importance of miR-21 plasma level as a diagnostic and prognostic biomarker for BC.

Abstract Image

查看原文本刊更多论文

微RNA表达在乳腺癌中的生物标志物作用。

背景：女性中最常见的癌症是乳腺癌（BC）。MicroRNA-21是首批在许多恶性肿瘤（包括胶质瘤、BC和结直肠癌）中发现水平升高的肿瘤化合物之一（miR-21）。MiRNA与细胞凋亡、侵袭、转移和增殖等过程有关，这些都是已知的癌症特征。本研究旨在探讨miR-21在BC中的分子基础和临床意义，因为microrna在BC中起着至关重要的作用。材料和方法：使用Taq-Man探针对MiR 21进行定量聚合酶链反应（qPCR）分析。热剖面包括在95°C下变性5 min，然后在95°C下变性20 s，在52°C下退火20 s，在72°C下延伸30 s的40个循环。然后进行实时聚合酶链反应和逆转录（RT）两个单独的运行。基于计算机断层扫描值的基因表达水平的相对定量（折叠变化）用于检查RT-qPCR中miR-21和甘油醛-3-磷酸脱氢酶（Livak和Schmittgen）的数据。结果：患者血清miR-21水平的平均倍数变化（8.17±2.49）明显高于对照组（1.02±0.23）。血清miR-21水平在III期、IV期和淋巴结的平均倍数变化明显高于I期和II期。血清miR-21基因表达的平均倍数变化随Ki67水平的变化而显著。结论：我们的研究结果证明了miR-21血浆水平作为BC的诊断和预后生物标志物的重要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Annals of African Medicine MEDICINE, GENERAL & INTERNAL-

CiteScore

0.90

自引率

0.00%

发文量

期刊介绍： The Annals of African Medicine is published by the Usmanu Danfodiyo University Teaching Hospital, Sokoto, Nigeria and the Annals of African Medicine Society. The Journal is intended to serve as a medium for the publication of research findings in the broad field of Medicine in Africa and other developing countries, and elsewhere which have relevance to Africa. It will serve as a source of information on the state of the art of Medicine in Africa, for continuing education for doctors in Africa and other developing countries, and also for the publication of meetings and conferences. The journal will publish articles I any field of Medicine and other fields which have relevance or implications for Medicine.