{"title":"\"Cell climbing stones\" - varying the surfaces of electrospun nanofibers with protrusions as secondary structures to manipulate neural cell behaviors.","authors":"Yawen Wang, Xiaopei Zhang, Lijie Yao, Yuying Yan, Yuanfei Wang, Tong Wu","doi":"10.1039/d5nh00295h","DOIUrl":null,"url":null,"abstract":"<p><p>Effective neural repair requires the precise regulation of neurite outgrowth and coordinated migration of neural stem cells (NSCs) and Schwann cells (SCs). The synergistic integration of topographical cues, chemical signals and electrical stimulation can significantly enhance this process, among which topographical modulation has emerged as a research focus due to its direct regulatory effects on cellular behavior. Micro/nanoscale topological features (nanogrooves and protrusions) can markedly promote neurite outgrowth and cell migration by matching the mechanical characteristics of growth cone filopodia (100-300 nm). Building upon our previous work, we designed a series of aligned nanofibers with SiO<sub>2</sub> protrusions of varying sizes and concentrations to mimic \"cell climbing stones,\" systematically investigating their regulatory effects on neurite growth, and the migration of NSCs and SCs. The results demonstrated that nanofibers with oriented protrusions as secondary structures significantly enhanced the motility of SCs and NSCs while promoting neurite extension. Notably, nanofibers fabricated with 200 nm SiO<sub>2</sub> nanoparticles blended with PCL at 6% SiO<sub>2</sub> concentration exhibited the most pronounced regulatory effects on neural cell behavior.</p>","PeriodicalId":93,"journal":{"name":"Nanoscale Horizons","volume":" ","pages":""},"PeriodicalIF":8.0000,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nanoscale Horizons","FirstCategoryId":"88","ListUrlMain":"https://doi.org/10.1039/d5nh00295h","RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}
引用次数: 0
Abstract
Effective neural repair requires the precise regulation of neurite outgrowth and coordinated migration of neural stem cells (NSCs) and Schwann cells (SCs). The synergistic integration of topographical cues, chemical signals and electrical stimulation can significantly enhance this process, among which topographical modulation has emerged as a research focus due to its direct regulatory effects on cellular behavior. Micro/nanoscale topological features (nanogrooves and protrusions) can markedly promote neurite outgrowth and cell migration by matching the mechanical characteristics of growth cone filopodia (100-300 nm). Building upon our previous work, we designed a series of aligned nanofibers with SiO2 protrusions of varying sizes and concentrations to mimic "cell climbing stones," systematically investigating their regulatory effects on neurite growth, and the migration of NSCs and SCs. The results demonstrated that nanofibers with oriented protrusions as secondary structures significantly enhanced the motility of SCs and NSCs while promoting neurite extension. Notably, nanofibers fabricated with 200 nm SiO2 nanoparticles blended with PCL at 6% SiO2 concentration exhibited the most pronounced regulatory effects on neural cell behavior.
期刊介绍:
Nanoscale Horizons stands out as a premier journal for publishing exceptionally high-quality and innovative nanoscience and nanotechnology. The emphasis lies on original research that introduces a new concept or a novel perspective (a conceptual advance), prioritizing this over reporting technological improvements. Nevertheless, outstanding articles showcasing truly groundbreaking developments, including record-breaking performance, may also find a place in the journal. Published work must be of substantial general interest to our broad and diverse readership across the nanoscience and nanotechnology community.