"Cell climbing stones" - varying the surfaces of electrospun nanofibers with protrusions as secondary structures to manipulate neural cell behaviors.

IF 8 2区 材料科学 Q1 CHEMISTRY, PHYSICAL
Yawen Wang, Xiaopei Zhang, Lijie Yao, Yuying Yan, Yuanfei Wang, Tong Wu
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引用次数: 0

Abstract

Effective neural repair requires the precise regulation of neurite outgrowth and coordinated migration of neural stem cells (NSCs) and Schwann cells (SCs). The synergistic integration of topographical cues, chemical signals and electrical stimulation can significantly enhance this process, among which topographical modulation has emerged as a research focus due to its direct regulatory effects on cellular behavior. Micro/nanoscale topological features (nanogrooves and protrusions) can markedly promote neurite outgrowth and cell migration by matching the mechanical characteristics of growth cone filopodia (100-300 nm). Building upon our previous work, we designed a series of aligned nanofibers with SiO2 protrusions of varying sizes and concentrations to mimic "cell climbing stones," systematically investigating their regulatory effects on neurite growth, and the migration of NSCs and SCs. The results demonstrated that nanofibers with oriented protrusions as secondary structures significantly enhanced the motility of SCs and NSCs while promoting neurite extension. Notably, nanofibers fabricated with 200 nm SiO2 nanoparticles blended with PCL at 6% SiO2 concentration exhibited the most pronounced regulatory effects on neural cell behavior.

“细胞攀岩石”——改变电纺丝纳米纤维表面的突起作为二级结构来操纵神经细胞的行为。
有效的神经修复需要精确调节神经突的生长和神经干细胞(NSCs)和雪旺细胞(SCs)的协调迁移。地形信号、化学信号和电刺激的协同整合可以显著增强这一过程,其中地形调制因其对细胞行为的直接调节作用而成为研究热点。微/纳米尺度的拓扑特征(纳米沟槽和突起)可以通过匹配生长锥丝状伪足(100-300 nm)的力学特征,显著促进神经突的生长和细胞迁移。在我们之前工作的基础上,我们设计了一系列具有不同大小和浓度的SiO2突出物的排列纳米纤维来模拟“细胞攀登石”,系统地研究了它们对神经突生长和NSCs和SCs迁移的调节作用。结果表明,纳米纤维定向突起作为二级结构可显著增强SCs和NSCs的运动能力,同时促进神经突的延伸。值得注意的是,200 nm SiO2纳米颗粒与6% SiO2浓度的PCL共混制备的纳米纤维对神经细胞行为的调节作用最为显著。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Nanoscale Horizons
Nanoscale Horizons Materials Science-General Materials Science
CiteScore
16.30
自引率
1.00%
发文量
141
期刊介绍: Nanoscale Horizons stands out as a premier journal for publishing exceptionally high-quality and innovative nanoscience and nanotechnology. The emphasis lies on original research that introduces a new concept or a novel perspective (a conceptual advance), prioritizing this over reporting technological improvements. Nevertheless, outstanding articles showcasing truly groundbreaking developments, including record-breaking performance, may also find a place in the journal. Published work must be of substantial general interest to our broad and diverse readership across the nanoscience and nanotechnology community.
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