{"title":"Enhancing Skin Repair and Photodamage Reversal With 755-nm Picosecond Laser and Bioactive Polymer Dots in a Nude Mouse Model.","authors":"Bing-Qi Wu, Yen-Jen Wang, Chang-Cheng Chang, Tzong-Yuan Juang, Hsiu-Mei Chiang, Yi-Hsuan Tu, Jia-Chee Siew, Siao-Cian Fan","doi":"10.1111/wrr.70069","DOIUrl":null,"url":null,"abstract":"<p><p>Picosecond laser technology has emerged as a promising intervention for reversing photodamage by promoting epidermal repair and collagen regeneration. Polymer dots (PDs) have shown antioxidant and wound-healing properties. This study evaluates the efficacy of a combined 755-nm picosecond laser and bioactive PDs treatment to enhance skin repair and reverse photodamage in a nude mouse model subjected to UVB irradiation. Using twelve 6-week-old BALB/c nude mice, the experiment was conducted over 10 weeks, with the mice assigned to one of four groups: UVB alone, UVB + PEG1000, UVB + PD + PEG1000 and UVB + Laser +PD + PEG1000. Assessment methods included immunohistochemistry, enzyme-linked immunosorbent assay and Masson's trichrome staining to evaluate collagen content, epidermal thickness and protein expression associated with skin repair. The UVB + Laser + PD + PEG1000 group achieved a significant reduction in epidermal thickness by Day 22 (p = 0.0017) and showed superior collagen retention compared to the UVB group (p < 0.0001). In addition, MMP-9 levels in the UVB + PD + PEG1000 group reduced by 4.2% on Day 11 versus 9.3% in the UVB-only group on Day 1 (p = 0.03), while IL-6 levels markedly decreased in all treated groups compared to the UVB group, indicating diminished inflammation (p < 0.001). Analysis of Smad2/3 signalling on Day 11 in the UVB + PD + PEG1000 group revealed enhanced activation of skin repair pathways, with values reaching 2.8% compared to 1.3% in the UVB group on Day 1 (p = 0.0026). These findings suggest that combining the 755-nm picosecond laser with bioactive PDs offers a novel therapeutic approach for photodamage repair, enhancing collagen synthesis and inflammation reduction and supporting further investigation into its potential in skin ageing and damage reversal.</p>","PeriodicalId":23864,"journal":{"name":"Wound Repair and Regeneration","volume":"33 4","pages":"e70069"},"PeriodicalIF":3.4000,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Wound Repair and Regeneration","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1111/wrr.70069","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Picosecond laser technology has emerged as a promising intervention for reversing photodamage by promoting epidermal repair and collagen regeneration. Polymer dots (PDs) have shown antioxidant and wound-healing properties. This study evaluates the efficacy of a combined 755-nm picosecond laser and bioactive PDs treatment to enhance skin repair and reverse photodamage in a nude mouse model subjected to UVB irradiation. Using twelve 6-week-old BALB/c nude mice, the experiment was conducted over 10 weeks, with the mice assigned to one of four groups: UVB alone, UVB + PEG1000, UVB + PD + PEG1000 and UVB + Laser +PD + PEG1000. Assessment methods included immunohistochemistry, enzyme-linked immunosorbent assay and Masson's trichrome staining to evaluate collagen content, epidermal thickness and protein expression associated with skin repair. The UVB + Laser + PD + PEG1000 group achieved a significant reduction in epidermal thickness by Day 22 (p = 0.0017) and showed superior collagen retention compared to the UVB group (p < 0.0001). In addition, MMP-9 levels in the UVB + PD + PEG1000 group reduced by 4.2% on Day 11 versus 9.3% in the UVB-only group on Day 1 (p = 0.03), while IL-6 levels markedly decreased in all treated groups compared to the UVB group, indicating diminished inflammation (p < 0.001). Analysis of Smad2/3 signalling on Day 11 in the UVB + PD + PEG1000 group revealed enhanced activation of skin repair pathways, with values reaching 2.8% compared to 1.3% in the UVB group on Day 1 (p = 0.0026). These findings suggest that combining the 755-nm picosecond laser with bioactive PDs offers a novel therapeutic approach for photodamage repair, enhancing collagen synthesis and inflammation reduction and supporting further investigation into its potential in skin ageing and damage reversal.
期刊介绍:
Wound Repair and Regeneration provides extensive international coverage of cellular and molecular biology, connective tissue, and biological mediator studies in the field of tissue repair and regeneration and serves a diverse audience of surgeons, plastic surgeons, dermatologists, biochemists, cell biologists, and others.
Wound Repair and Regeneration is the official journal of The Wound Healing Society, The European Tissue Repair Society, The Japanese Society for Wound Healing, and The Australian Wound Management Association.