Streptozotocin induced hyperglycemia in the axolotl.

IF 1.5 3区 生物学 Q2 ANATOMY & MORPHOLOGY
Pernille Lajer Sørensen, Anita Dittrich, Henrik Lauridsen
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引用次数: 0

Abstract

Background: Diabetes is a group of diseases characterized by loss of β cell mass and/or function, resulting in hyperglycemia. With no established curative treatment, this has initiated research in β cell regeneration. Current animal models have either limited regenerative capacity (mice) or small size and evolutionary distance from humans (zebrafish). There is a need for new models to study endogenous regeneration pathways. This study proposes the axolotl salamander (Ambystoma mexicanum) as a model for studying the regeneration of β cells and aims to establish a protocol for STZ-induced hyperglycemia to mimic a diabetic state.

Results: In this pilot study, five streptozotocin (STZ) protocols were tested, and the most effective one was identified on the basis of glucose tolerance tests. Blood glucose levels were monitored to track both disease progression and remission. Histological examination of the pancreas and systemic effects of STZ treatment were also evaluated.

Conclusion: Induction of a diabetes-like state (hyperglycemia) in axolotls was possible with STZ, but variability among animals suggests the need for a higher degree of normalization or larger sample sizes. Histological regeneration was not observed, though blood glucose levels normalized over time. Some STZ-treated animals developed edema, but its cause remains unknown.

链脲佐菌素诱导美西鲀高血糖。
背景:糖尿病是一组以β细胞质量和/或功能丧失为特征的疾病,导致高血糖。由于没有确定的治疗方法,这已经启动了β细胞再生的研究。目前的动物模型要么再生能力有限(小鼠),要么体型小,与人类进化距离远(斑马鱼)。需要新的模型来研究内源性再生途径。本研究将蝾螈(Ambystoma mexicanum)作为研究β细胞再生的模型,旨在建立stz诱导的高血糖模拟糖尿病状态的方案。结果:本试验试验了5种链脲佐菌素(STZ)方案,通过糖耐量试验确定了最有效的方案。监测血糖水平以跟踪疾病进展和缓解情况。胰腺组织学检查和STZ治疗的全身效果也进行了评估。结论:STZ可能诱导蝾螈出现糖尿病样状态(高血糖),但动物之间的差异表明需要更高程度的归一化或更大的样本量。虽然血糖水平随时间恢复正常,但没有观察到组织学再生。一些stz治疗的动物出现水肿,但其原因尚不清楚。
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来源期刊
Developmental Dynamics
Developmental Dynamics 生物-发育生物学
CiteScore
5.10
自引率
8.00%
发文量
116
审稿时长
3-8 weeks
期刊介绍: Developmental Dynamics, is an official publication of the American Association for Anatomy. This peer reviewed journal provides an international forum for publishing novel discoveries, using any model system, that advances our understanding of development, morphology, form and function, evolution, disease, stem cells, repair and regeneration.
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