Biological behavior of human gingival fibroblasts and formation of microbial biofilm on 3D-printed dental resin restorations.

IF 6.3 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Dental Materials Pub Date : 2025-07-21 DOI:10.1016/j.dental.2025.07.012

Benedetta Ghezzi, Lorenza Artesani, Laura Giovati, Simonetta Croci, Francesca Valotti, Giovanni Mergoni, Maddalena Manfredi

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引用次数: 0

Abstract

Objectives: This study aimed to investigate the interaction dynamics among two commercially available 3D printed resins (V and R) presenting different surface topographies and human gingival fibroblasts, as well as oral microorganisms.

Methods: 3D-printed samples of two commercial resins underwent various polishing treatments. Surfaces characteristics and biological interactions were analyzed with contact angle measurements, scanning electron microscopy (SEM) and atomic force microscopy (AFM), cellular viability assays, and quantitative real-time PCR (qRT-PCR). The formation of mono- and polymicrobial biofilms of Streptococcus sanguinis and Candida albicans on the resins was evaluated through SEM and confocal laser scanning microscopy (CLSM).

Results: AFM and SEM analyses revealed significant differences in surface roughness and hydrophilicity among the treatments. Both the resins demonstrated good biocompatibility, however one altered fibroblastic morphology, a finding supported by the differential expression of ITα-6 and IL-6 genes. Variations in the total biomass of S. sanguinis and C. albicans were observed between untreated controls and treated surfaces.

Significance: The V resin exhibited superior performance in reducing microbial adhesion and promoting favorable interactions with human gingival fibroblasts in its commercial form, outperforming R resin.

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人牙龈成纤维细胞的生物学行为及3d打印牙科树脂修复体上微生物生物膜的形成。

目的：研究两种不同表面形貌的3D打印树脂（V和R）与人类牙龈成纤维细胞以及口腔微生物之间的相互作用动力学。方法：两种商用树脂的3d打印样品进行不同的抛光处理。通过接触角测量、扫描电镜（SEM）和原子力显微镜（AFM）、细胞活力测定和实时荧光定量PCR （qRT-PCR）分析表面特性和生物相互作用。通过扫描电镜（SEM）和共聚焦激光扫描显微镜（CLSM）研究了树脂上血链球菌和白色念珠菌单、多微生物生物膜的形成情况。结果：AFM和SEM分析显示不同处理的表面粗糙度和亲水性有显著差异。两种树脂均表现出良好的生物相容性，但其中一种树脂改变了成纤维细胞的形态，这一发现得到了ITα-6和IL-6基因表达差异的支持。在未处理的对照和处理过的表面之间观察到血链球菌和白色念珠菌总生物量的变化。意义：在商业形态下，V树脂在减少微生物粘附和促进与人牙龈成纤维细胞良好相互作用方面表现出优越的性能，优于R树脂。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Dental Materials 工程技术-材料科学：生物材料

CiteScore

9.80

自引率

10.00%

发文量

290

审稿时长

67 days

期刊介绍： Dental Materials publishes original research, review articles, and short communications. Academy of Dental Materials members click here to register for free access to Dental Materials online. The principal aim of Dental Materials is to promote rapid communication of scientific information between academia, industry, and the dental practitioner. Original Manuscripts on clinical and laboratory research of basic and applied character which focus on the properties or performance of dental materials or the reaction of host tissues to materials are given priority publication. Other acceptable topics include application technology in clinical dentistry and dental laboratory technology. Comprehensive reviews and editorial commentaries on pertinent subjects will be considered.