LC-MS/MS-guided separation of guaiacolane-type sesquiterpenes with anti-inflammatory activities from Chrysanthemum indicum†

IF 4.6 3区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

RSC Advances Pub Date : 2025-07-22 DOI:10.1039/D5RA03586D

Xinyue Li, Yanfen Cai, Yunshuang Hu, Limei Miu, Yanyu Zhang, Shiyun Huang, Min Wei, Qing Ma, Zhongqiu Liu, Hua Zhou and Peng Wu

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Abstract

Four previously undescribed compounds, including three guaiacane-type sesquiterpenoids (1–3) and one oleanane-type triterpenoid (4), along with seven known sesquiterpenoids, were isolated from the aerial parts of Chrysanthemum indicum using LC-MS/MS-guided fractionation. Structures were elucidated by IR, UV, HR-ESI-MS, and 1D/2D NMR analyses, and their absolute configurations were determined by ECD calculations. The anti-inflammatory activity was evaluated in LPS-stimulated RAW264.7 cells with NF-κB translocation by high-content imaging (HCI). Compound 2 reduced NF-κB translocation (IC₅₀ = 9.70 μM) without cytotoxicity at 20 μM. Compounds 8 and 9 showed potent activity (IC₅₀ = 2.04 and 1.21 μM, respectively) and no cytotoxicity at 6.25 μM.

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hplc - ms / ms引导分离菊花中具有抗炎活性的愈创木酚烷型倍半萜

采用液相色谱-质谱联用技术，从菊花的地上部分分离得到了4个已知的倍半萜类化合物，包括3个愈创木烷型倍半萜类化合物（1-3）和1个齐墩烷型倍半萜类化合物(4)。通过IR， UV， HR-ESI-MS和1D/2D NMR分析对其结构进行了鉴定，并通过ECD计算确定了其绝对构型。采用高含量显像（HCI）评价lps刺激的NF-κB易位RAW264.7细胞的抗炎活性。化合物2降低NF-κB易位（IC50 = 9.70 μM），在20 μM时无细胞毒性。化合物8和9的IC50分别为2.04和1.21 μM，且在6.25 μM下无细胞毒性。

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来源期刊

RSC Advances chemical sciences-

CiteScore

7.50

自引率

2.60%

发文量

3116

审稿时长

1.6 months

期刊介绍： An international, peer-reviewed journal covering all of the chemical sciences, including multidisciplinary and emerging areas. RSC Advances is a gold open access journal allowing researchers free access to research articles, and offering an affordable open access publishing option for authors around the world.