Anoxia-responsive microRNA profile of freshwater turtle red skeletal muscle

IF 0.9 Q4 GENETICS & HEREDITY

Gene Reports Pub Date : 2025-07-21 DOI:10.1016/j.genrep.2025.102302

Tighe Bloskie, Kenneth B. Storey

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Abstract

The red-eared slider (Trachemys scripta elegans) is a uniquely impressive vertebrate facultative anaerobe, capable of 18 weeks without oxygen at 3 °C. Metabolic rate depression (∼85 %) is the core feature of anaerobiosis and is characterized by the suppression of costly processes like protein synthesis/decay and the cell cycle. The elucidation of microRNA (miRNA) action in support of animal extreme stress adaptation is increasing, but is currently lacking in T.s. elegans anoxia tolerance. Here, we use small RNA sequencing and subsequent bioinformatic analyses to identify differentially expressed miRNA and predicted target pathways in 20 h anoxic red skeletal muscle of red-eared slider turtles. Of the 52 mapped miRNA species, we identify two that were upregulated (miR-2114-5p, let-7f-5p) and two that were downregulated (miR-1260b, miR-5100) under 20 h anoxic conditions (|FC| > 1.5; p < 0.05). KEGG and GO analysis predict miRNA contribute to the aerobic to anaerobic respiration shift and outline miRNA-mediated inhibition of numerous gene sets in (1) protein turnover, (2) RNA turnover and (3) the cell cycle. Conversely, alleviated miRNA interference in branched amino acid biosynthesis, arachidonic acid and linolenic acid metabolism suggest a role in atrophy resistance of skeletal muscles.

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淡水龟红骨骼肌缺氧反应的microRNA谱

红耳滑鼠（Trachemys scripta elegans）是一种独特的兼性厌氧脊椎动物，能够在3°C的无氧环境下存活18周。代谢率下降（~ 85%）是厌氧症的核心特征，其特征是抑制蛋白质合成/衰变和细胞周期等昂贵过程。支持动物极端应激适应的microRNA （miRNA）作用的阐明越来越多，但目前在秀丽隐杆线虫的缺氧耐受中尚缺乏。在这里，我们使用小RNA测序和随后的生物信息学分析来鉴定红耳滑龟20 h缺氧红色骨骼肌中差异表达的miRNA并预测目标通路。在52个已定位的miRNA物种中，我们确定了两个在20小时缺氧条件下上调（miR-2114-5p, let-7f-5p）和两个下调（miR-1260b, miR-5100） (|FC| >；1.5;p & lt;0.05)。KEGG和GO分析预测miRNA有助于有氧呼吸到无氧呼吸的转变，并概述了miRNA介导的对(1)蛋白质转换、(2)RNA转换和(3)细胞周期中许多基因集的抑制。相反，miRNA对支链氨基酸生物合成、花生四烯酸和亚麻酸代谢的干扰减轻，表明其在骨骼肌抗萎缩中起作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

7.70%

发文量

246

审稿时长

49 days

期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.