Generation of a human induced pluripotent stem cell reporter line to investigate cell division and proliferation

IF 0.7 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Stem cell research Pub Date : 2025-07-16 DOI:10.1016/j.scr.2025.103776

Alessia Costa , Ayse Boese , Elisa Mohr , Carla Borisch , Hannah Jill Hunkler , Jeannine Hoepfner , Shambhabi Chatterjee , Thomas Thum , Christian Bär

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引用次数: 0

Abstract

Understanding cell division in disease contexts is of paramount importance for elucidating disease mechanisms and developing regenerative therapies, such as cardiac regeneration. Nevertheless, tools for identifying and to studying key factors in regenerative processes in human cells remain scarce. Here, we generated a human induced pluripotent stem cell (hiPSC) reporter line expressing a cell cycle-regulated cyclinB1-eGFP construct that enables live tracking of proliferating human cells. The reporter hiPSC line successfully differentiated into cardiomyocytes (CMs), endothelial cells (ECs), and fibroblasts (FBs), with eGFP⁺ cells identifying actively dividing populations across lineages. Each cell type exhibited appropriate lineage-specific marker expression and high differentiation efficiency. Importantly, the cyclinB1-eGFP system allowed real-time identification and tracking of proliferating (eGFP⁺) cells within these differentiated populations. This tool provides an innovative platform for screening potential pro-proliferative compounds, facilitating the discovery of novel therapies to stimulate or inhibit cell division.

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人诱导多能干细胞报告系的生成，用于研究细胞分裂和增殖

了解疾病背景下的细胞分裂对于阐明疾病机制和发展再生疗法（如心脏再生）至关重要。然而，用于识别和研究人类细胞再生过程中关键因素的工具仍然很少。在这里，我们产生了一个人类诱导多能干细胞（hiPSC）报告系，表达细胞周期调节的cyclinB1-eGFP结构，能够实时跟踪增殖的人类细胞。报告细胞hiPSC系成功分化为心肌细胞（CMs）、内皮细胞（ECs）和成纤维细胞（FBs）， eGFP+细胞在谱系中识别出活跃的分裂群体。每种细胞类型均表现出适当的谱系特异性标记表达和高分化效率。重要的是，cyclinB1-eGFP系统允许实时识别和跟踪这些分化群体中的增殖（eGFP+）细胞。该工具为筛选潜在的促增殖化合物提供了一个创新的平台，促进了刺激或抑制细胞分裂的新疗法的发现。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

期刊介绍： Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.