Concentrations of serum amyloid A and haptoglobin in mare blood and milk as indicators of subclinical mastitis

IF 2.5 2区农林科学 Q3 REPRODUCTIVE BIOLOGY

Theriogenology Pub Date : 2025-07-17 DOI:10.1016/j.theriogenology.2025.117591

Dominika Domańska , Olga Witkowska- Piłaszewicz , Michał Trela , Karol Pawłowski , Bartosz Pawliński , Malgorzata Domino

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引用次数: 0

Abstract

Mastitis is an emerging health concern in lactating mares, particularly given the growing commercial interest in mare's milk. This study evaluated the diagnostic potential of acute phase proteins (APPs), specifically serum amyloid A (SAA), milk amyloid A (MAA), and haptoglobin (Hp), measured in blood and milk as indicators of clinical and subclinical mastitis. Twenty-four Thoroughbred mares were prospectively monitored during lactation and weaning. Plasma and milk samples were collected and analyzed using commercial ELISA kits.

Plasma SAA concentrations were significantly higher in mares with clinical mastitis compared to healthy (p < 0.001) and subclinically affected mares (p < 0.01), particularly on day 1 postpartum. In contrast, plasma SAA concentrations remained low 181–183 days after foaling, even in mares with subclinical mastitis. Milk MAA and Hp concentrations were significantly elevated in mares with clinical (p < 0.001) and subclinical mastitis (p < 0.05) compared to healthy mares. MAA concentrations were higher in subclinical mastitis detected during weaning (SM II group) than postpartum (SM I group) (p < 0.05).

A moderate positive correlation between MAA and plasma Hp concentrations was observed in clinical mastitis cases (r = 0.57, p < 0.01), suggesting concurrent systemic and local responses, while weak correlations were found in subclinical and healthy mares.

These findings suggest that plasma SAA is a useful marker of systemic inflammation in clinical mastitis, whereas MAA and milk Hp are more sensitive markers of local mammary gland inflammation, particularly in subclinical cases. Further research with larger populations is warranted to define diagnostic thresholds and explore APP dynamics throughout lactation.

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血清淀粉样蛋白A和接触珠蛋白在马血和奶中的浓度作为亚临床乳腺炎的指标

乳腺炎是哺乳期母马的一个新兴健康问题，特别是考虑到对母马奶日益增长的商业兴趣。本研究评估了急性期蛋白（APPs）的诊断潜力，特别是血清淀粉样蛋白A （SAA）、乳淀粉样蛋白A （MAA）和触珠蛋白（Hp），在血液和乳中测量，作为临床和亚临床乳腺炎的指标。24匹纯种母马在哺乳期和断奶期进行前瞻性监测。血浆和牛奶样品采集和分析使用商用ELISA试剂盒。患有临床乳腺炎的母马血浆SAA浓度显著高于健康母马(p <；0.001)和亚临床感染的母马(p <；0.01)，尤其是产后第1天。相比之下，即使在患有亚临床乳腺炎的母马中，产驹后181-183天血浆SAA浓度仍然很低。马乳MAA和Hp浓度显著升高，临床(p <；0.001)和亚临床乳腺炎(p <；与健康母马相比，0.05)。断奶期（SM II组）亚临床乳腺炎患者MAA浓度高于产后（SM I组）(p <；0.05)。临床乳腺炎患者的MAA与血浆Hp浓度呈正相关(r = 0.57, p <；0.01)，提示全身和局部反应同时发生，而在亚临床和健康母马中发现弱相关性。这些发现表明，血浆SAA是临床乳腺炎全身性炎症的有用标志物，而MAA和乳汁Hp是局部乳腺炎症的更敏感标志物，特别是在亚临床病例中。有必要在更大的人群中进行进一步的研究，以确定诊断阈值并探索整个哺乳期的APP动态。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Theriogenology 农林科学-生殖生物学

CiteScore

5.50

自引率

14.30%

发文量

387

审稿时长

72 days

期刊介绍： Theriogenology provides an international forum for researchers, clinicians, and industry professionals in animal reproductive biology. This acclaimed journal publishes articles on a wide range of topics in reproductive and developmental biology, of domestic mammal, avian, and aquatic species as well as wild species which are the object of veterinary care in research or conservation programs.