Evaluation of Erythroid Maturation Patterns in Unexplained anemia in the Elderly.

IF 0.6 4区医学 Q4 HEMATOLOGY

Indian Journal of Hematology and Blood Transfusion Pub Date : 2025-07-01 Epub Date: 2024-10-29 DOI:10.1007/s12288-024-01879-8

Shrinza Gupta, Mrinalini Kotru, Ashish Goel, Richa Gupta

{"title":"Evaluation of Erythroid Maturation Patterns in Unexplained anemia in the Elderly.","authors":"Shrinza Gupta, Mrinalini Kotru, Ashish Goel, Richa Gupta","doi":"10.1007/s12288-024-01879-8","DOIUrl":null,"url":null,"abstract":"In the elderly, a sizeable proportion of anemia remains unexplained. Erythroid maturation patterns may provide clues to underlying pathophysiology. Hence, this study was conducted to identify unique erythroid maturation patterns in unexplained anemia (UA) and their usefulness in providing a clue to the underlying cause of anemia. Immunophenotyping by flow cytometry was performed on bone marrow (BM) aspirates. The maturation patterns of erythroid precursors were studied, and erythroid cells were classified into early erythroid precursors (CD34+/CD117+/CD71-), proerythroblasts (CD45neg-dim/CD71+/CD117+/CD235a-), erythroblasts (CD117-/CD71+/CD235a+) and mature erythrocytes (CD235a+). Three different patterns were observed based on CD71 expression (heterogeneous, homogeneous, and negative to dim), the percentage of CD117 + erythroid cells, and MFI CD71 (increased or decreased). Three of these cases also showed mild CD117+/CD235a + copositive aberrant/asynchronous pattern. UA cases in the elderly demonstrated three different maturation patterns. These patterns need to be validated along with a cytogenetic work-up in further studies with larger sample size and longer follow-ups to gain a better understanding of the progression of UA.Supplementary information: The online version contains supplementary material available at 10.1007/s12288-024-01879-8.","PeriodicalId":49188,"journal":{"name":"Indian Journal of Hematology and Blood Transfusion","volume":"41 3","pages":"571-578"},"PeriodicalIF":0.6000,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12267786/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indian Journal of Hematology and Blood Transfusion","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s12288-024-01879-8","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/10/29 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"HEMATOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

In the elderly, a sizeable proportion of anemia remains unexplained. Erythroid maturation patterns may provide clues to underlying pathophysiology. Hence, this study was conducted to identify unique erythroid maturation patterns in unexplained anemia (UA) and their usefulness in providing a clue to the underlying cause of anemia. Immunophenotyping by flow cytometry was performed on bone marrow (BM) aspirates. The maturation patterns of erythroid precursors were studied, and erythroid cells were classified into early erythroid precursors (CD34+/CD117+/CD71-), proerythroblasts (CD45^neg-dim/CD71+/CD117+/CD235a-), erythroblasts (CD117-/CD71+/CD235a+) and mature erythrocytes (CD235a+). Three different patterns were observed based on CD71 expression (heterogeneous, homogeneous, and negative to dim), the percentage of CD117 + erythroid cells, and MFI CD71 (increased or decreased). Three of these cases also showed mild CD117+/CD235a + copositive aberrant/asynchronous pattern. UA cases in the elderly demonstrated three different maturation patterns. These patterns need to be validated along with a cytogenetic work-up in further studies with larger sample size and longer follow-ups to gain a better understanding of the progression of UA.

Supplementary information: The online version contains supplementary material available at 10.1007/s12288-024-01879-8.

查看原文本刊更多论文

老年人不明原因性贫血中红细胞成熟模式的评价。

在老年人中，相当大比例的贫血仍无法解释。红系成熟模式可能为潜在的病理生理学提供线索。因此，本研究旨在确定不明原因贫血（UA）中独特的红细胞成熟模式，并为贫血的潜在病因提供线索。用流式细胞术对骨髓（BM）抽吸物进行免疫分型。研究了红细胞前体的成熟模式，将红细胞分为早期红细胞前体（CD34+/CD117+/CD71-）、原红细胞（cd45negi -dim/CD71+/CD117+/CD235a-）、成红细胞（CD117-/CD71+/CD235a+）和成熟红细胞（CD235a+）。基于CD71表达（异质性、同质性和阴性到暗淡）、CD117 +红系细胞的百分比和MFI CD71（增加或减少），观察到三种不同的模式。其中3例还表现出轻微的CD117+/CD235a +合成畸变/非同步模式。老年UA病例表现出三种不同的成熟模式。这些模式需要在更大样本量和更长时间随访的进一步研究中与细胞遗传学检查一起进行验证，以更好地了解UA的进展。补充资料：在线版本提供补充资料，网址为10.1007/s12288-024-01879-8。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Indian Journal of Hematology and Blood Transfusion HEMATOLOGY-

CiteScore

1.70

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Indian Journal of Hematology and Blood Transfusion is a medium for propagating and exchanging ideas within the medical community. It publishes peer-reviewed articles on a variety of aspects of clinical hematology, laboratory hematology and hemato-oncology. The journal exists to encourage scientific investigation in the study of blood in health and in disease; to promote and foster the exchange and diffusion of knowledge relating to blood and blood-forming tissues; and to provide a forum for discussion of hematological subjects on a national scale. The Journal is the official publication of The Indian Society of Hematology & Blood Transfusion.