Administration of adipose tissue-derived mesenchymal stem cells and bone marrow-derived mesenchymal stem cells in anosmic mice.

IF 1 4区 医学 Q2 MEDICINE, GENERAL & INTERNAL
Turkish Journal of Medical Sciences Pub Date : 2025-04-05 eCollection Date: 2025-01-01 DOI:10.55730/1300-0144.6028
Burak Hazir, Alper Ceylan, Emin Ümit Bağriaçik, Duygu Dayanir, Betül Öğüt, Nihan Örüklü, Mücahit Yalçin, Berkay Şimşek, Muammer Melih Şahin
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Abstract

Background/aim: Anosmia, a serious condition that affects the sense of smell, has no universally agreed-upon treatment. Adult stem cells are considered a potential option for treating anosmia. This study aims to evaluate the efficacy of mesenchymal stem cells derived from different tissues in an anosmic mouse model induced by 3-methylindole (3-MI).

Materials and methods: In our study, 36 mice with 3-MI-induced anosmia were divided into subgroups. Anosmia was confirmed by performing a food-finding test (FFT) in each group. Intranasal phosphate-buffered saline was administered to the first group, adipose tissue-derived mesenchymal stem cells (ADSCs) to the second group, and bone marrow-derived mesenchymal stem cells (BMSCs) to the third group. Stem cells were obtained from green fluorescent protein (GFP)-transgenic mice. Olfactory function was evaluated weekly using the FFTs. Mice were sacrificed at the second and fourth weeks following 3-MI injection and examined histopathologically.

Results: Compared to the control group, stem cell-transplanted groups demonstrated significantly improved food-finding times measured at week 2 and week 4 FFTs following the 3-MI injection (respectively; p = 0.001, p = 0.008). Additionally, increased olfactory marker protein expression and olfactory epithelial thickness, along with reduced epithelial damage, were observed in the stem cell-transplanted groups compared to the control group (p < 0.05). Histologically, BMSCs showed greater efficacy than ADSCs in promoting olfactory regeneration. Furthermore, GFP+ cells were detected in the olfactory epithelium and olfactory bulbs of the stem cell-transplanted groups.

Conclusion: It was observed that intranasally transplanted stem cells could reach the damaged olfactory region and enhance olfactory regeneration and functional recovery. Both ADSCs and BMSCs were effective in treatment and appear to be promising therapeutic modalities.

脂肪组织来源的间充质干细胞和骨髓来源的间充质干细胞在嗅觉缺失小鼠中的应用。
背景/目的:嗅觉缺失是一种影响嗅觉的严重疾病,目前尚无普遍认可的治疗方法。成体干细胞被认为是治疗嗅觉缺失的潜在选择。本研究旨在评估来自不同组织的间充质干细胞在3-甲基吲哚(3-MI)诱导的嗅觉小鼠模型中的作用。材料与方法:将36只3- mi致嗅觉缺失小鼠分为亚组。通过对每组进行食物寻找测试(FFT)来确认嗅觉缺失。第一组使用鼻内磷酸盐缓冲盐水,第二组使用脂肪组织源性间充质干细胞(ADSCs),第三组使用骨髓源性间充质干细胞(BMSCs)。从绿色荧光蛋白(GFP)转基因小鼠获得干细胞。每周使用fft对嗅觉功能进行评估。3-MI注射后第2周和第4周处死小鼠,进行组织病理学检查。结果:与对照组相比,干细胞移植组在3-MI注射后第2周和第4周的FFTs(分别;P = 0.001, P = 0.008)。此外,与对照组相比,干细胞移植组嗅觉标记蛋白表达增加,嗅上皮厚度增加,上皮损伤减轻(p < 0.05)。组织学上,骨髓间充质干细胞比ADSCs在促进嗅觉再生方面表现出更大的功效。干细胞移植组嗅上皮和嗅球中均检测到GFP+细胞。结论:鼻内移植干细胞能到达受损嗅觉区,促进嗅觉再生和功能恢复。ADSCs和BMSCs在治疗中都是有效的,似乎是有希望的治疗方式。
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来源期刊
Turkish Journal of Medical Sciences
Turkish Journal of Medical Sciences 医学-医学:内科
CiteScore
4.60
自引率
4.30%
发文量
143
审稿时长
3-8 weeks
期刊介绍: Turkish Journal of Medical sciences is a peer-reviewed comprehensive resource that provides critical up-to-date information on the broad spectrum of general medical sciences. The Journal intended to publish original medical scientific papers regarding the priority based on the prominence, significance, and timeliness of the findings. However since the audience of the Journal is not limited to any subspeciality in a wide variety of medical disciplines, the papers focusing on the technical  details of a given medical  subspeciality may not be evaluated for publication.
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