Rolling circle amplification/transcription-based nanotechnology for efficient delivery of nucleic acid drugs

IF 6.6 2区材料科学 Q1 CHEMISTRY, PHYSICAL

Nanoscale Horizons Pub Date : 2025-07-08 DOI:10.1039/D5NH00364D

Xun You, Qingxuan Zeng, Tianshuang Xia, Xiaocui Guo, Chi Yao and Dayong Yang

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引用次数: 0

Abstract

Rolling circle amplification/transcription (RCA/RCT) nanotechnology offers a breakthrough platform for nucleic acid drug delivery, leveraging enzymatically produced ultra-long, programmable nucleic acid chains to engineer multifunctional nanostructures. In this review, we give an overview of RCA/RCT-based nanocarriers for nucleic acid drug delivery, systematically summarizing their key design aspects: (1) nanoization strategies through biomineralization, electrostatic compression, nanomaterial-assisted assembly and base pairing/entanglement; (2) drug loading approaches via design on template, complementary base pairing and electrostatic binding; (3) targeting modalities including aptamers, proteins, polymers and small molecule ligands; and (4) controlled release mechanisms responsive to endogenous/exogenous enzymes and intracellular microenvironments. We showcase their significant therapeutic advances in gene therapy, immunotherapy, and combination therapy. This overview provides critical insights for developing next-generation RCA/RCT delivery platforms to address pressing biomedical needs.

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基于滚动圈扩增/转录的纳米技术用于核酸药物的有效递送。

滚动环扩增/转录（RCA/RCT）纳米技术为核酸药物递送提供了一个突破性的平台，利用酶促产生的超长可编程核酸链来设计多功能纳米结构。本文综述了基于RCA/ rct的核酸药物递送纳米载体的研究进展，系统总结了它们的主要设计要点：(1)通过生物矿化、静电压缩、纳米材料辅助组装和碱基配对/纠缠实现纳米化策略；(2)模板设计、互补碱基配对设计和静电结合设计；(3)靶向方式，包括适体、蛋白质、聚合物和小分子配体；(4)内源性/外源性酶和细胞内微环境的调控释放机制。我们展示了他们在基因治疗、免疫治疗和联合治疗方面的重大治疗进展。本综述为开发下一代RCA/RCT输送平台以满足迫切的生物医学需求提供了重要见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nanoscale Horizons Materials Science-General Materials Science

CiteScore

16.30

自引率

1.00%

发文量

141

期刊介绍： Nanoscale Horizons stands out as a premier journal for publishing exceptionally high-quality and innovative nanoscience and nanotechnology. The emphasis lies on original research that introduces a new concept or a novel perspective (a conceptual advance), prioritizing this over reporting technological improvements. Nevertheless, outstanding articles showcasing truly groundbreaking developments, including record-breaking performance, may also find a place in the journal. Published work must be of substantial general interest to our broad and diverse readership across the nanoscience and nanotechnology community.