A Comparative Analysis of Antimicrobial Resistance Patterns and Genes in Staphylococcus aureus From Humans and Animals in Veterinary Clinics Across Thailand
{"title":"A Comparative Analysis of Antimicrobial Resistance Patterns and Genes in Staphylococcus aureus From Humans and Animals in Veterinary Clinics Across Thailand","authors":"Shutipen Buranasinsup, Anuwat Wiratsudakul, Sarin Suwanpakdee, Sineenard Jiemtaweeboon, Khuanwalai Maklon, Walasinee Sakcamduang, Boonrat Chantong","doi":"10.1155/tbed/5541655","DOIUrl":null,"url":null,"abstract":"<div>\n <p><b>Background:</b> Antimicrobial resistance (AMR) in <i>Staphylococcus aureus</i> (<i>S. aureus</i>) poses critical public health challenges by limiting treatment efficacy and elevating morbidity, mortality, and healthcare costs.</p>\n <p><b>Methods:</b> This study examined the prevalence and characteristics of AMR in <i>S. aureus</i> isolated from humans (veterinarians, veterinary assistants, and pet owners) and animals (dogs and cats) in veterinary clinics across five provinces in Thailand. A total of 882 samples were collected from which 188 <i>S. aureus</i> isolates were recovered and subjected to antimicrobial susceptibility testing and resistance gene detection.</p>\n <p><b>Results:</b> Substantial variations in AMR profiles were observed across host categories, with veterinarians and veterinary assistants exhibiting higher resistance rates than pet owners. The β-lactam resistance gene <i>bla</i>Z was prevalent in all groups, whereas <i>mec</i>A was predominantly detected in veterinarians and dogs, emphasizing the occupational risk and zoonotic transmission potential. The aminoglycoside resistance gene <i>aac</i>A<i>–aph</i>D was common in cats, and quinolone resistance genes <i>gyr</i>A and <i>grl</i>A were identified in veterinarians and dogs. Macrolide resistance genes <i>msr</i>A and <i>erm</i>A, lincosamide resistance gene <i>lin</i>A, and tetracycline resistance gene <i>tet</i>K were widely distributed across the groups. <i>Agr</i> typing of <i>S. aureus</i> isolates revealed diverse group distributions, with <i>agr</i> group I was predominant in human samples and associated with the highest AMR gene expression, while <i>agr</i> group III was most prevalent in animal samples and also exhibited elevated AMR gene expression within that group.</p>\n <p><b>Conclusions:</b> This study underscored the diverse distribution of AMR genes, with veterinarians and veterinary assistants facing higher occupational risks. The findings highlighted the importance of integrated antimicrobial stewardship and surveillance within a One Health framework to mitigate the spread of AMR in veterinary and community settings.</p>\n </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2025 1","pages":""},"PeriodicalIF":3.0000,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/tbed/5541655","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Transboundary and Emerging Diseases","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1155/tbed/5541655","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Antimicrobial resistance (AMR) in Staphylococcus aureus (S. aureus) poses critical public health challenges by limiting treatment efficacy and elevating morbidity, mortality, and healthcare costs.
Methods: This study examined the prevalence and characteristics of AMR in S. aureus isolated from humans (veterinarians, veterinary assistants, and pet owners) and animals (dogs and cats) in veterinary clinics across five provinces in Thailand. A total of 882 samples were collected from which 188 S. aureus isolates were recovered and subjected to antimicrobial susceptibility testing and resistance gene detection.
Results: Substantial variations in AMR profiles were observed across host categories, with veterinarians and veterinary assistants exhibiting higher resistance rates than pet owners. The β-lactam resistance gene blaZ was prevalent in all groups, whereas mecA was predominantly detected in veterinarians and dogs, emphasizing the occupational risk and zoonotic transmission potential. The aminoglycoside resistance gene aacA–aphD was common in cats, and quinolone resistance genes gyrA and grlA were identified in veterinarians and dogs. Macrolide resistance genes msrA and ermA, lincosamide resistance gene linA, and tetracycline resistance gene tetK were widely distributed across the groups. Agr typing of S. aureus isolates revealed diverse group distributions, with agr group I was predominant in human samples and associated with the highest AMR gene expression, while agr group III was most prevalent in animal samples and also exhibited elevated AMR gene expression within that group.
Conclusions: This study underscored the diverse distribution of AMR genes, with veterinarians and veterinary assistants facing higher occupational risks. The findings highlighted the importance of integrated antimicrobial stewardship and surveillance within a One Health framework to mitigate the spread of AMR in veterinary and community settings.
期刊介绍：
Transboundary and Emerging Diseases brings together in one place the latest research on infectious diseases considered to hold the greatest economic threat to animals and humans worldwide. The journal provides a venue for global research on their diagnosis, prevention and management, and for papers on public health, pathogenesis, epidemiology, statistical modeling, diagnostics, biosecurity issues, genomics, vaccine development and rapid communication of new outbreaks. Papers should include timely research approaches using state-of-the-art technologies. The editors encourage papers adopting a science-based approach on socio-economic and environmental factors influencing the management of the bio-security threat posed by these diseases, including risk analysis and disease spread modeling. Preference will be given to communications focusing on novel science-based approaches to controlling transboundary and emerging diseases. The following topics are generally considered out-of-scope, but decisions are made on a case-by-case basis (for example, studies on cryptic wildlife populations, and those on potential species extinctions):
Pathogen discovery: a common pathogen newly recognised in a specific country, or a new pathogen or genetic sequence for which there is little context about — or insights regarding — its emergence or spread.
Prevalence estimation surveys and risk factor studies based on survey (rather than longitudinal) methodology, except when such studies are unique. Surveys of knowledge, attitudes and practices are within scope.
Diagnostic test development if not accompanied by robust sensitivity and specificity estimation from field studies.
Studies focused only on laboratory methods in which relevance to disease emergence and spread is not obvious or can not be inferred (“pure research” type studies).
Narrative literature reviews which do not generate new knowledge. Systematic and scoping reviews, and meta-analyses are within scope.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
