Agarose-loaded mesoporous silica nanocomposite embedded in a polyether sulfone substrate for thin film solid-phase microextraction: An approach for narcotic drugs extraction from eye tears in a multi-channel microfluidic device prior to liquid chromatography-tandem mass spectrometry

IF 5.6 1区 化学 Q1 CHEMISTRY, ANALYTICAL
Shirin Yadegari , Shahram Seidi , Reyhaneh Namdari
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Abstract

Herein, a solid-phase microextraction method was developed based on an agarose-loaded mesoporous silica nanocomposite embedded in a polyether sulfone thin film, followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. The microextraction was performed using a multi-channel microfluidic device incorporating the prepared nanocomposite thin film to extract morphine (MOR), heroin (HER), codeine (COD), and methamphetamine (MET) from eye tear samples. Field emission scanning electron microscopy (FE-SEM), Fourier-transform infrared spectroscopy (FT-IR), Brunauer–Emmett–Teller (BET), thermal gravimetric analysis (TGA), and transmission electron microscopy (TEM) were employed to characterize the nanocomposite. The influence of various parameters on the extraction efficiency—including sample pH, sample flow rate, nanoparticle and polyether sulfone amounts, eluent type and volume, and eluent flow rate—was systematically studied. Under optimized conditions, the limits of detection (LOD) were 0.07 and 0.06 ng mL−1 for COD and HER, respectively, and 0.1 ng mL−1 for MET and MOR. The matrix effect, ranging from 8.85 % to 45.64 %, was evaluated using box-whisker plots. Intra-day and inter-day RSDs% were below 4.5 % and 4.0 %, respectively. Calibration curves showed linearity over 0.5–80.0 ng mL−1 for MET, 0.2–80.0 ng mL−1 for COD, and 0.4–80.0 ng mL−1 for MOR and HER. Relative recovery (RR%) values assessing accuracy in tear samples ranged from 90.0 % to 112.0 %. Greenness and Blueness scores of 0.72 and 57.5, respectively, demonstrate that the suggested approach is both sustainable and applicable. These findings indicate that this method is a suitable alternative for measuring trace amounts of narcotic drugs in tear samples.

Abstract Image

嵌入聚醚砜衬底的琼脂糖负载介孔二氧化硅纳米复合材料用于薄膜固相微萃取:一种液相色谱-串联质谱法前的多通道微流体装置从泪液中提取麻醉药物的方法
本研究基于负载琼脂糖的介孔二氧化硅纳米复合材料包埋在聚醚砜薄膜上,采用液相色谱-串联质谱(LC-MS/MS)分析,建立了固相微萃取方法。采用多通道微流控装置对制备的纳米复合薄膜进行微萃取,从泪液样品中提取吗啡(MOR)、海洛因(HER)、可待因(COD)和甲基苯丙胺(MET)。采用场发射扫描电镜(FE-SEM)、傅里叶变换红外光谱(FT-IR)、布鲁诺尔-埃米特-泰勒(BET)、热重分析(TGA)和透射电子显微镜(TEM)对纳米复合材料进行了表征。系统研究了样品pH、样品流速、纳米颗粒和聚醚砜用量、洗脱液种类和体积、洗脱液流速等参数对萃取效率的影响。在优化条件下,COD和HER的检出限分别为0.07和0.06 ng mL−1,MET和MOR的检出限分别为0.1 ng mL−1。利用盒晶须图对基质效应进行了评价,其范围为8.85% ~ 45.64%。日内rsd < 4.5%,日内rsd < 4.0%。MET在0.5-80.0 ng mL - 1范围内呈线性,COD在0.2-80.0 ng mL - 1范围内呈线性,MOR和HER在0.4-80.0 ng mL - 1范围内呈线性。相对恢复(RR%)值评估泪液样本的准确性范围为90.0%至112.0%。绿度和蓝度得分分别为0.72和57.5,表明该方法具有可持续性和适用性。这些结果表明,该方法是测定泪液样品中微量麻醉药物的一种合适的替代方法。
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来源期刊
Talanta
Talanta 化学-分析化学
CiteScore
12.30
自引率
4.90%
发文量
861
审稿时长
29 days
期刊介绍: Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.
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