Heparin-loaded silk fibroin microparticles/bacterial nanocellulose (Hep@SFMPs/BNC) conduits for application as small-caliber artificial blood vessels

IF 10.7 1区 化学 Q1 CHEMISTRY, APPLIED
Geli Li , Luhan Bao , Lin Chen , Xingping Zhou , Feng F. Hong
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Abstract

Small-caliber artificial blood vessels are highly demanded and face challenges, including thrombosis and intimal hyperplasia. The excellent properties of bacterial nanocellulose (BNC) make it an excellent material for preparing artificial blood vessels. Heparin (Hep)-loaded silk fibroin microparticles (SFMPs) were synthesized in situ within the conduit wall via liquid pressure injection and phase separation, aiming to improve BNC's anticoagulant properties. SFMPs served as a carrier for Hep, enabling its sustained slow release from the BNC conduits, and the Hep@SFMPs/BNC promoted the adhesion and proliferation of vascular cells. In vitro blood experiments and gene expression analysis of HUVECs showed that the composite conduits had a decreased coagulation rate, fewer platelets adsorbed, and lower levels of inflammation compared with BNC conduits. After 5 months of abdominal aortic replacement in rats, an in situ formation of a continuous endothelial layer was observed, covering 90 % of the surface with endothelial cells. The proposed preparation method is simple, efficient, and requires fewer reagents, with the added benefit of eliminating the need for crosslinking agents. The Hep@SFMPs/BNC conduit has the characteristics of anticoagulant and endothelial growth-promoting properties, with strong evidence from animal experiments supporting the potential of Hep@SFMPs/BNC conduits as small-caliber artificial blood vessels.

Abstract Image

载肝素丝素微粒/细菌纳米纤维素(Hep@SFMPs/BNC)用于小口径人工血管的导管
小口径人造血管的需求量很大,面临血栓形成和内膜增生等挑战。细菌纳米纤维素(BNC)的优异性能使其成为制备人造血管的优良材料。通过液相压力注射和相分离,在导管壁内原位合成了负载肝素(Hep)的丝素蛋白微粒(SFMPs),以提高BNC的抗凝血性能。SFMPs作为Hep的载体,使其从BNC导管中持续缓慢释放,Hep@SFMPs/BNC促进血管细胞的粘附和增殖。体外血液实验和HUVECs基因表达分析表明,与BNC导管相比,复合导管凝血率降低,血小板吸附量减少,炎症水平降低。在大鼠腹主动脉置换5个月后,观察到原位形成连续的内皮层,内皮细胞覆盖了90%的表面。所提出的制备方法简单,高效,所需试剂较少,并且省去了交联剂的额外好处。Hep@SFMPs/BNC导管具有抗凝血和促进内皮细胞生长的特性,动物实验有力地支持了Hep@SFMPs/BNC导管作为小口径人工血管的潜力。
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来源期刊
Carbohydrate Polymers
Carbohydrate Polymers 化学-高分子科学
CiteScore
22.40
自引率
8.00%
发文量
1286
审稿时长
47 days
期刊介绍: Carbohydrate Polymers stands as a prominent journal in the glycoscience field, dedicated to exploring and harnessing the potential of polysaccharides with applications spanning bioenergy, bioplastics, biomaterials, biorefining, chemistry, drug delivery, food, health, nanotechnology, packaging, paper, pharmaceuticals, medicine, oil recovery, textiles, tissue engineering, wood, and various aspects of glycoscience. The journal emphasizes the central role of well-characterized carbohydrate polymers, highlighting their significance as the primary focus rather than a peripheral topic. Each paper must prominently feature at least one named carbohydrate polymer, evident in both citation and title, with a commitment to innovative research that advances scientific knowledge.
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