Isolation and molecular characterization of antibiotic resistance determinants amongst Klebsiella quasipneumoniae recovered from poultry farm: an eco-health implication

Veterinary Vaccine Pub Date : 2025-07-03 DOI:10.1016/j.vetvac.2025.100133

J.P. Ebhonu , B.E. Igere , E.O. Odjadjare , C.I. Igeleke

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Abstract

Klebsiella species belong to a group of well-studied human pathogen with emerging global reports on antimicrobial resistance and hyper-virulent clones. Its occurrence has been reported with high abundance in diverse niches (environmental nexus) including poultry farm and may constitute a reservoir of genetic elements and strains transmission. Its hyper-virulent character has aroused public health concern which necessitates study. The study investigates isolation and molecular characterization of antibiotic resistance determinants amongst klebsiella sp recovered from poultry farm and their potential eco-health implications. Samples were collected from poultry farms including Ugbor (poultry A), Sapele road nexus (poultry B), Ekenwan region (poultry C) and Evboriaria region (poultry D) between August and October 2020. Recovered isolates were analysed using standard microbiological, veterinary antibiotic susceptibility testing (CLSI-VAST) standard guideline and molecular biology techniques. Observed results showed that the mean total heterotrophic bacterial counts for feeds ranged from 18.0 × 10⁵ ± 3.04 (poultry C) to 28.2 × 10⁵ ± 1.55 cfu/g (poultry B) while Shigella-Salmonella counts ranged from 2.32 × 10³ ± 0.84 (Poultry C) to 8.30 × 10³ ± 1.27 cfu/g (poultry A). The mean total heterotrophic bacterial counts for water-samples ranged from 0.85 × 10⁵ ± 0.49 (poultry A) to 1.85 × 10⁵ ± 0.35 cfu/ml (poultry B). The mean total heterotrophic counts for poultry dung ranged from 11.9 × 10⁵ ± 2.96 (poultry C) to 36.4 ± 4.17 cfu/g (poultry B). Following the AST screening, it was revealed that all isolates recovered were resistant to Ceftazidime, Cefixime and Cefuroxime with an apparent resistance to Gentamicin, Augumentin, Ciprofloxacin and Ofloxacin and 100 % susceptible to Nitrofurantoin. The 16S rRNA sequencing analysis showed 100 % similarity of two strains as Klebsiella quasipneumoniae implicating the poultry farm as potential dissemination hub for such hyper-virulent and multiple-antibiotic-resistant (MAR) phenotype and genotype in the environment which may serve as cross-contamination subjects of MAR strains during processing and distribution of products.

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从家禽养殖场恢复的准肺炎克雷伯菌中抗生素耐药性决定因素的分离和分子特征：生态健康意义

克雷伯氏菌属一组研究充分的人类病原体，全球正在出现抗微生物药物耐药性和高毒性克隆的报告。据报道，它在包括家禽养殖场在内的不同生态位（环境关系）中发生率很高，可能构成遗传元件和菌株传播的储存库。它的高毒性引起了公众的关注，有必要对其进行研究。本研究调查了从家禽养殖场回收的克雷伯氏菌中抗生素耐药性决定因素的分离和分子特征及其潜在的生态健康意义。样本采集于2020年8月至10月期间，包括Ugbor（家禽A）、Sapele road nexus（家禽B）、Ekenwan地区（家禽C）和Evboriaria地区（家禽D）的家禽养殖场。采用标准微生物学、兽医抗生素药敏试验（CLSI-VAST）标准指南和分子生物学技术对回收的分离株进行分析。结果表明：饲料中异养细菌总数平均值为18.0 × 105±3.04（禽C） ~ 28.2 × 105±1.55 cfu/g（禽B），志贺氏沙门氏菌总数平均值为2.32 × 103±0.84（禽C） ~ 8.30 × 103±1.27 cfu/g（禽A）。水样中异养细菌总数平均值为0.85 × 105±0.49（家禽A） ~ 1.85 × 105±0.35 cfu/ml（家禽B）。禽粪平均总异养计数范围为11.9 × 105±2.96 (C) ~ 36.4±4.17 (B) cfu/g。经AST筛选，所有分离株对头孢他啶、头孢克肟和头孢呋辛均耐药，对庆大霉素、奥古汀、环丙沙星和氧氟沙星均有明显耐药，对呋喃妥因100%敏感。16S rRNA测序分析显示，两株菌株与准肺炎克雷伯菌相似度为100%，表明该家禽养殖场是该高毒多重耐药（MAR）表型和基因型的潜在传播中心，在产品加工和分销过程中可能成为MAR菌株的交叉污染对象。

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Veterinary Vaccine

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