Transcriptomic analysis reveals the immunomodulatory role of Clostridium autoethanogenum protein on Litopenaeus vannamei under variable salinity conditions

IF 6.1 1区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Jian Chen, Hongming Wang, Hang Yuan, Naijie Hu, Yudong Zheng, Yonghua Hu, Shuang Zhang
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引用次数: 0

Abstract

Salinity is a critical environmental factor in aquaculture, and Clostridium autoethanogenum protein (CAP) shows potential as a new feed protein source. This 8-week study compared two diets (fish meal [FM] and CAP) for juvenile Pacific white shrimp (Litopenaeus vannamei) at salinity levels of 15‰, 30‰, and 45‰. A total of 720 L. vannamei with an initial average body weight of 0.38 ± 0.01 g were randomly assigned to six experimental groups, each with three biological replicates of 40 shrimp per replicate. The study examined CAP’s impact on shrimp growth, immune response, and transcriptome, using two-way ANOVA to analyze the results. The results indicated that compared to the FM group, the shrimp in the CAP group exhibited a significantly higher weight gain rate and specific growth rate at the same salinity (P < 0.05). However, at 45‰ salinity, the shrimp in the CAP group had a higher feed conversion ratio and feed intake compared to the FM group (P < 0.05). After infection with white spot syndrome virus, the CAP group exhibited a significantly higher survival rate at 15‰ and 45‰ salinity compared to the FM group (P < 0.05). As salinity increased, most immune enzyme activities and gene expression levels in the FM group initially increased and then decreased (P < 0.05). Under the same salinity, except for phenoloxidase activity which showed no significant difference at 45‰ salinity, all other immune-related indicators and gene expressions in the CAP group were significantly higher than those in the FM group (P < 0.05). Transcriptome analysis revealed that the differentially expressed genes (DEGs) between FM and CAP groups at various salinity levels were primarily associated with immune and metabolic pathways. Additionally, by combining the analysis of these DEGs with immune-related indicators, it was observed that under different salinity conditions, CAP was associated with the co-expression of immune and metabolism-related genes as well as changes in enzyme activity. In summary, CAP as the main protein source boosts growth, disease resistance, and nonspecific immunity in L. vannamei, while also regulating immune enzyme activity and gene expression to improve adaptability to salinity changes.
转录组学分析揭示了自产乙醇梭状芽胞杆菌蛋白在变盐度条件下对凡纳滨对虾的免疫调节作用
盐度是水产养殖的重要环境因子,自产乙醇梭菌蛋白(Clostridium autoethangenum protein, CAP)具有作为一种新型饲料蛋白来源的潜力。本研究对凡纳滨对虾(Litopenaeus vannamei)幼虾在盐度为15‰、30‰和45‰时的两种饲料(鱼粉[FM]和CAP)进行了比较。选取初始平均体重为0.38±0.01 g的凡纳滨对虾720尾,随机分为6个试验组,每组设3个生物重复,每个重复40尾。该研究检查了CAP对虾生长、免疫反应和转录组的影响,使用双向方差分析分析结果。结果表明,与FM组相比,相同盐度下,CAP组对虾的增重率和特定生长率显著高于FM组(P <;0.05)。但在45‰盐度下,CAP组对虾的饲料系数和采食量均高于FM组(P <;0.05)。感染白斑综合征病毒后,CAP组在15‰和45‰盐度下的存活率显著高于FM组(P <;0.05)。随着盐度的升高,FM组大部分免疫酶活性和基因表达水平先升高后降低(P <;0.05)。在相同盐度下,除酚氧化酶活性在45‰盐度下差异不显著外,CAP组其他免疫相关指标和基因表达量均显著高于FM组(P <;0.05)。转录组分析显示,不同盐度水平下FM组和CAP组之间的差异表达基因(DEGs)主要与免疫和代谢途径相关。此外,通过对这些deg与免疫相关指标的分析,我们发现在不同盐度条件下,CAP与免疫和代谢相关基因的共表达以及酶活性的变化有关。综上所述,CAP作为主要蛋白质来源,促进了凡纳梅的生长、抗病性和非特异性免疫,同时调节免疫酶活性和基因表达,提高对盐度变化的适应性。
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来源期刊
Animal Nutrition
Animal Nutrition Agricultural and Biological Sciences-Animal Science and Zoology
CiteScore
7.40
自引率
3.20%
发文量
172
审稿时长
12 weeks
期刊介绍: Animal Nutrition encompasses the full gamut of animal nutritional sciences and reviews including, but not limited to, fundamental aspects of animal nutrition such as nutritional requirements, metabolic studies, body composition, energetics, immunology, neuroscience, microbiology, genetics and molecular and cell biology related to nutrition, and more applied aspects of animal nutrition, such as raw material evaluation, feed additives, nutritive value of novel ingredients and feed safety.
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