Application of Herbal-Based Antioxidants in Human Sperm Cryopreservation: A Systematic Review and Meta-Analysis Study.

IF 1.6 4区生物学

Biopreservation and Biobanking Pub Date : 2025-07-18 DOI:10.1177/19475535251359868

Vahid Shokri-Asl, Rasa Khodavirdilou, Hamed Hajipour, Maryam Pashaiasl, Amir Fattahi

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Abstract

Sperm cryopreservation (SC) is an acceptable laboratory procedure for long-term storage of sperm. However, this procedure causes sperm damage. This review aimed to systematically investigate the effects of herbal-based antioxidants (HBAs) application on sperm parameters during SC. Following determination of the main keywords and searching strategy, various databases were searched systematically. Primary and secondary screenings were applied based on inclusion/exclusion criteria. Finally, 27 randomized controlled trial studies using 15 different HBAs and involving 557 normal and 130 abnormal semen samples were included for the meta-analysis. HBAs administration before or during the SC process improved total motility (mean difference [MD]: -6.31 [95% confidence interval [CI]: -10.27, -2.34], p < 0.05), viability (MD: -1.21 [95% CI: -1.52, -0.89], p < 0.001), morphology (MD: -1.72 [95% CI: -2.89, -0.54], p < 0.05), reactive oxygen species (H₂O₂) (MD: 7.58 [95% CI: 3.90, 11.26], p < 0.001), DNA integrity (MD: -13.21 [95% CI: -19.94, -6.49], p < 0.001), and sperm DNA fragmentation (SDF) (MD: 3.76 [95% CI: 2.38, 5.14], p < 0.001) after thawing in normal specimens. In abnormal semen samples, the HBAs improved viability (MD: -8.54 [95% CI: -11.18, -5.19], p < 0.001), progressive motility (MD: -4.55 [95% CI: -7.37, -1.73], p < 0.05) and ameliorated SDF (MD: 3.76 [95% CI: 1.74, 5.79], p < 0.001). Also, HBAs had no effects on total motility (MD: -0.08 [95% CI: -3.29, 3.14], p > 0.05), and morphology (MD: -0.31 [95% CI: -0.71, 0.09], p > 0.05). Application of HBAs in cryomedia before and during SC can improve sperm parameters (including viability, motility, and morphology), decrease oxidative stress and SDF levels.

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草药抗氧化剂在人类精子冷冻保存中的应用：系统综述和荟萃分析研究。

精子冷冻保存（SC）是一种可接受的精子长期储存的实验室程序。然而，这个过程会导致精子受损。本文旨在系统探讨中药抗氧化剂（HBAs）的应用对SC过程中精子参数的影响。在确定主要关键词和检索策略的基础上，系统检索了各种数据库。根据纳入/排除标准进行初级和次级筛查。最后，使用15种不同HBAs的27项随机对照试验纳入meta分析，涉及557例正常和130例异常精液样本。在SC过程之前或期间给药HBAs改善了总运动能力（平均差[MD]: -6.31[95%可信区间[CI]: -10.27, -2.34], p < 0.05）、活力（MD: -1.21 [95% CI: -1.52, -0.89], p < 0.001）、形态学（MD: -1.72 [95% CI: -2.89, -0.54], p < 0.05）、活性氧（H2O2）（MD: 7.58 [95% CI: 3.90, 11.26], p < 0.001）、DNA完整性（MD: -13.21 [95% CI: -19.94, -6.49], p < 0.001）和精子DNA片段化（SDF）（MD: 3.76 [95% CI: -19.94, -6.49], p < 0.001）。（2.38, 5.14）， p < 0.001)。在异常精液样本中，HBAs改善了活力（MD: -8.54 [95% CI: -11.18, -5.19], p < 0.001），进行性运动（MD: -4.55 [95% CI: -7.37, -1.73], p < 0.05）和改善了SDF （MD: 3.76 [95% CI: 1.74, 5.79], p < 0.001）。此外，HBAs对总运动（MD: -0.08 [95% CI: -3.29, 3.14], p > 0.05）和形态学（MD: -0.31 [95% CI: -0.71, 0.09], p > 0.05）没有影响。在SC前和SC过程中在低温培养基中应用HBAs可以改善精子参数（包括活力、活力和形态），降低氧化应激和SDF水平。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biopreservation and Biobanking Biochemistry, Genetics and Molecular Biology-General Biochemistry,Genetics and Molecular Biology

自引率

12.50%

发文量

114

期刊介绍： Biopreservation and Biobanking is the first journal to provide a unifying forum for the peer-reviewed communication of recent advances in the emerging and evolving field of biospecimen procurement, processing, preservation and banking, distribution, and use. The Journal publishes a range of original articles focusing on current challenges and problems in biopreservation, and advances in methods to address these issues related to the processing of macromolecules, cells, and tissues for research. In a new section dedicated to Emerging Markets and Technologies, the Journal highlights the emergence of new markets and technologies that are either adopting or disrupting the biobank framework as they imprint on society. The solutions presented here are anticipated to help drive innovation within the biobank community. Biopreservation and Biobanking also explores the ethical, legal, and societal considerations surrounding biobanking and biorepository operation. Ideas and practical solutions relevant to improved quality, efficiency, and sustainability of repositories, and relating to their management, operation and oversight are discussed as well.