{"title":"High-throughput measurement of Drosophila feeding behavior.","authors":"Huai-Zheng Zheng, Shuo Song, Jing-Hao Tang, Hong-Shan Liu, Man Song, Zhen-Xia Chen","doi":"10.1016/j.crmeth.2025.101109","DOIUrl":null,"url":null,"abstract":"<p><p>Accurate measurement of Drosophila feeding is vital for metabolic and aging studies, but current assays lack the throughput and sensitivity needed for large-scale screens. We introduce FlyPlate-BCA, a 96-well platform that combines automated single-fly tracking with BCA protein quantification for high-resolution, longitudinal intake measurements. FlyPlate-BCA detects a 0.1× nutrient dilution with 90% power using just four replicates, three times fewer than the capillary feeder (CAFE) assay. It uncovers sucrose-driven hyperphagia independent of osmotic stress and preserved lipid-satiety responses, while extended monitoring reveals a female-specific metabolic adaptation. By delivering individual-level data at scale, FlyPlate-BCA establishes a benchmark for nutritional genomics and appetite neurobiology and streamlines nutraceutical and genetic screening.</p>","PeriodicalId":29773,"journal":{"name":"Cell Reports Methods","volume":" ","pages":"101109"},"PeriodicalIF":4.5000,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12461584/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cell Reports Methods","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1016/j.crmeth.2025.101109","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/7/18 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0
Abstract
Accurate measurement of Drosophila feeding is vital for metabolic and aging studies, but current assays lack the throughput and sensitivity needed for large-scale screens. We introduce FlyPlate-BCA, a 96-well platform that combines automated single-fly tracking with BCA protein quantification for high-resolution, longitudinal intake measurements. FlyPlate-BCA detects a 0.1× nutrient dilution with 90% power using just four replicates, three times fewer than the capillary feeder (CAFE) assay. It uncovers sucrose-driven hyperphagia independent of osmotic stress and preserved lipid-satiety responses, while extended monitoring reveals a female-specific metabolic adaptation. By delivering individual-level data at scale, FlyPlate-BCA establishes a benchmark for nutritional genomics and appetite neurobiology and streamlines nutraceutical and genetic screening.
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