Synergistic Interactions of Oxidized Amylose Silver Nanoparticles and Topically Applicable Antibiotics Against Staphylococcus aureus.

IF 2.6 3区生物学 Q3 MICROBIOLOGY

Current Microbiology Pub Date : 2025-07-19 DOI:10.1007/s00284-025-04380-1

Mehmet Erkan Çelebi, Umut Yılmaz, Sema Bagdat, Gülhan Vardar Unlu

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引用次数: 0

Abstract

Staphylococcus aureus causes serious infections that are increasingly difficult to treat due to antibiotic resistance. In this study, we synthesized oxidized amylose silver nanoparticles (OAAgNPs) and evaluated their synergistic potential with topical antibiotics against multidrug-resistant (MDR) S. aureus. OAAgNPs were synthesized and characterized using spectroscopic and microscopic techniques, confirming their nanoscale structure (5-10 nm) and chemical composition. Antibacterial testing against 50 clinical MDR isolates showed that OAAgNPs alone inhibited bacterial growth. More importantly, combinations with gentamicin (GEN), ciprofloxacin, mupirocin (MUP), or fusidic acid (FA) significantly enhanced antibacterial efficacy. Synergy was strongest with GEN and consistently observed with MUP and FA-antibiotics for which such nanoparticle synergy has rarely been documented. Cytotoxicity assays on mammalian cells demonstrated low toxicity at therapeutically relevant concentrations. Overall, this study highlights the potential of OAAgNP-antibiotic combinations as a promising strategy for managing MDR skin infections. Further research toward clinical formulation development is warranted.

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氧化直链银纳米颗粒与局部适用抗生素对金黄色葡萄球菌的协同作用。

金黄色葡萄球菌引起严重感染，由于抗生素耐药性，这种感染越来越难以治疗。在这项研究中，我们合成了氧化直链淀粉纳米银（OAAgNPs），并评估了它们与局部抗生素对抗多药耐药（MDR）金黄色葡萄球菌的协同潜力。合成了OAAgNPs，并利用光谱学和显微技术对其进行了表征，确定了OAAgNPs的纳米结构（5 ~ 10 nm）和化学成分。对50株临床MDR菌株的抑菌试验表明，OAAgNPs单独抑制细菌生长。更重要的是，与庆大霉素（GEN）、环丙沙星、莫匹罗星（MUP）或夫西地酸（FA）联合使用可显著提高抗菌效果。与GEN的协同作用最强，与MUP和fa -抗生素的协同作用一致，这种纳米颗粒的协同作用很少有文献记载。对哺乳动物细胞的细胞毒性试验表明，在治疗相关浓度下毒性较低。总的来说，这项研究强调了oaagnp -抗生素联合治疗耐多药皮肤感染的潜力。进一步的临床配方开发研究是必要的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current Microbiology 生物-微生物学

CiteScore

4.80

自引率

3.80%

发文量

380

审稿时长

2.5 months

期刊介绍： Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.