Utility of mast cell P815 for evaluating FcεRI-dependent and FcεRI-independent allergic effects.

IF 2.1 4区 医学 Q3 ALLERGY
Allergologia et immunopathologia Pub Date : 2025-07-01 eCollection Date: 2025-01-01 DOI:10.15586/aei.v53i4.1264
Ryosuke Misawa, Kazuhito Takemoto, Masaki Iji, Luo Hao, Akari Koresawa, Hiroyuki Watanabe
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引用次数: 0

Abstract

Introduction and objectives: The degranulation and release of inflammatory mediators mediated by the high-affinity immunoglobulin E (IgE) receptor (FcεRI) on mast cells in response to allergen contact is the driving force of anaphylaxis. This study shows that P815 cells, which were previously thought not to express FcεRI, cause a reaction similar to FcεRI-mediated degranulation in the presence of antigen and IgE.

Materials and methods: The kinetics of degranulation were evaluated by comparing P815 cells with FcεRI-expressing a rat basophilic leukemia (RBL-2H3) (that typically indicates the specific clone or subline within that cell line) cell lines. Degranulation activity was measured using the release rate of β-hexosaminidase as an indicator. P815 cells showed significant degranulation when compound 48/80 or anti-dinitrophenyl (DNP)-IgE antibody and DNP-human serum albumin (HSA) antigen were added simultaneously. Gene expression analysis confirmed the expression of each FcεRI subunit-specifically, the γ subunit expressed markedly. Moreover, the expression of the phosphorylation enzymes Lyn, spleen tyrosine kinase (Syk), Fyn, and Bruton tyrosine kinase (Btk), which are involved in degranulation, was upregulated.

Results: FcεRI has three subunits: α, β, and γ. P815 cells do not express FcεRI because they have messenger ribonucleic acid (mRNA) for the γ subunit but not for the α and β subunits. However, P815 expressed each subunit protein (α, β, and γ), as detected in the western blotting analysis of cell extracts in the presence of DNP-HSA antigen and anti-DNP-IgE.

Conclusion: These results suggest that P815 may cause degranulation via FcεRI. Therefore, P815 is considered to be a cell model that can evaluate both FcεRI-mediated and FcεRI-independent degranulation reactions in response to allergens.

肥大细胞P815在评价fc ε ri依赖性和fc ε ri非依赖性过敏反应中的应用。
简介和目的:高亲和力免疫球蛋白E (IgE)受体(FcεRI)介导肥大细胞对过敏原接触的脱颗粒和释放炎症介质是过敏反应的驱动力。本研究表明,先前认为不表达FcεRI的P815细胞在抗原和IgE存在下引起类似于FcεRI介导的脱粒反应。材料和方法:通过比较P815细胞与表达大鼠嗜碱性白血病(RBL-2H3)(通常表示该细胞系中的特定克隆或亚系)的fcε ri细胞系来评估脱颗粒动力学。以β-己糖氨酸酶释放率为指标测定脱粒活性。同时添加化合物48/80或抗二硝基苯(DNP)-IgE抗体和DNP-人血清白蛋白(HSA)抗原时,P815细胞出现明显的脱粒现象。基因表达分析证实各FcεRI亚基均有表达,其中γ亚基表达显著。此外,参与脱粒的磷酸化酶Lyn、脾酪氨酸激酶(Syk)、Fyn和布鲁顿酪氨酸激酶(Btk)的表达上调。结果:FcεRI具有α、β、γ三个亚基。P815细胞不表达FcεRI,因为它们具有γ亚基的信使核糖核酸(mRNA),而不具有α和β亚基。然而,在存在DNP-HSA抗原和抗dnp - ige的细胞提取物的western blotting分析中,P815表达了每个亚基蛋白(α, β和γ)。结论:P815可能通过fc - ε - ri引起脱粒。因此,P815被认为是一种既能评估fcε ri介导的脱粒反应,又能评估fcε ri不依赖的脱粒反应的细胞模型。
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来源期刊
CiteScore
3.70
自引率
0.00%
发文量
131
审稿时长
6-12 weeks
期刊介绍: Founded in 1972 by Professor A. Oehling, Allergologia et Immunopathologia is a forum for those working in the field of pediatric asthma, allergy and immunology. Manuscripts related to clinical, epidemiological and experimental allergy and immunopathology related to childhood will be considered for publication. Allergologia et Immunopathologia is the official journal of the Spanish Society of Pediatric Allergy and Clinical Immunology (SEICAP) and also of the Latin American Society of Immunodeficiencies (LASID). It has and independent international Editorial Committee which submits received papers for peer-reviewing by international experts. The journal accepts original and review articles from all over the world, together with consensus statements from the aforementioned societies. Occasionally, the opinion of an expert on a burning topic is published in the "Point of View" section. Letters to the Editor on previously published papers are welcomed. Allergologia et Immunopathologia publishes 6 issues per year and is included in the major databases such as Pubmed, Scopus, Web of Knowledge, etc.
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