IFN-γ cocultured mesenchymal stem cells promote substantial immunomodulatory effects in mice models of allergic asthma.

IF 2.1 4区 医学 Q3 ALLERGY
Allergologia et immunopathologia Pub Date : 2025-07-01 eCollection Date: 2025-01-01 DOI:10.15586/aei.v53i4.1348
Tao Chen, Jiaojiao Hu, Yanping Shi, Xia Li, Yujun Wang, Seyyed Shamsadin Athari, Entezar Mehrabi Nasab, Jingning Guo
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引用次数: 0

Abstract

Asthma is a debilitating lung disease characterized by airway inflammation and airflow obstruction. Immune cells, particularly T helper 2 (Th2) lymphocytes, are central players in the pathogenesis of asthma and mesenchymal stem cells (MSCs) have shown the capability of softening pathological inflammatory responses in asthma. Hence, we researched the immunopathologic effects MSCs cocultured with interferon (IFN)-γ, the main Th1 cytokine, in asthmatic mice. After isolation, MSCs were cocultured with IFN-γ and administered to asthmatic mice. Subsequent analyses included enumeration of broncho-alveolar lavage (BAL) fluid's inflammatory cells, determination of the levels of immunoglobulin E (IgE), leukotrienes (LTs), cytokines, chemokines, and histopathology assessment. The administration of IFN-γ-cocultured MSCs reduced the percentage of eosinophils in the BAL fluid and levels of IgE, LTs, cytokines, and chemokines. Also, there was a decrease in the eosinophilic infiltration of perivascular areas and periairways. IFN-γ cocultured MSCs could modulate immune responses and harness pathological events in allergic asthma.

IFN-γ共培养间充质干细胞促进过敏性哮喘小鼠模型的实质性免疫调节作用。
哮喘是一种以气道炎症和气流阻塞为特征的使人衰弱的肺部疾病。免疫细胞,尤其是辅助性T 2 (Th2)淋巴细胞,是哮喘发病机制中的核心角色,间充质干细胞(MSCs)已显示出软化哮喘病理性炎症反应的能力。因此,我们研究了MSCs与主要Th1细胞因子干扰素(IFN)-γ共培养对哮喘小鼠的免疫病理作用。分离后,MSCs与IFN-γ共培养并给予哮喘小鼠。随后的分析包括支气管肺泡灌洗液(BAL)炎症细胞的计数,免疫球蛋白E (IgE)、白三烯(lt)、细胞因子、趋化因子水平的测定和组织病理学评估。IFN-γ-共培养MSCs降低了BAL液中嗜酸性粒细胞的百分比以及IgE、lt、细胞因子和趋化因子的水平。同时,血管周围和气道周围嗜酸性粒细胞浸润减少。IFN-γ共培养MSCs可调节过敏性哮喘的免疫反应和病理事件。
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来源期刊
CiteScore
3.70
自引率
0.00%
发文量
131
审稿时长
6-12 weeks
期刊介绍: Founded in 1972 by Professor A. Oehling, Allergologia et Immunopathologia is a forum for those working in the field of pediatric asthma, allergy and immunology. Manuscripts related to clinical, epidemiological and experimental allergy and immunopathology related to childhood will be considered for publication. Allergologia et Immunopathologia is the official journal of the Spanish Society of Pediatric Allergy and Clinical Immunology (SEICAP) and also of the Latin American Society of Immunodeficiencies (LASID). It has and independent international Editorial Committee which submits received papers for peer-reviewing by international experts. The journal accepts original and review articles from all over the world, together with consensus statements from the aforementioned societies. Occasionally, the opinion of an expert on a burning topic is published in the "Point of View" section. Letters to the Editor on previously published papers are welcomed. Allergologia et Immunopathologia publishes 6 issues per year and is included in the major databases such as Pubmed, Scopus, Web of Knowledge, etc.
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