Ion release from bioactive dental liner materials by ion chromatography.

IF 6.3 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Dental Materials Pub Date : 2025-07-17 DOI:10.1016/j.dental.2025.07.008

Florian Konstantin Stangl, Karin Christine Huth, Christof Högg

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引用次数: 0

Abstract

Objective: Bioactive liner materials are used to promote dentine bridge formation, reduce early pulp inflammation, and support remineralization. Ion release is a key factor in understanding their role in biomineralization processes. This study is the first to employ a single method to quantitatively and qualitatively analyze the simultaneous release of multiple ions from various liner materials.

Methods: Ion chromatography was used to measure the release of 16 anions and cations into water, along with pH changes, on days 1, 4, 7, 14, and 30. The tested liner materials included an experimental liner/base from Solventum (EXP), TheraCal LC (TC), Dycal (DY), Lime-Lite Enhanced (LL), Fuji Lining LC Paste Pak (FL), and Medcem MTA (MC). Statistical analysis included the Shapiro-Wilk, Kruskal-Wallis, and Friedman-tests (α = 0.05).

Results: DY exhibited the highest pH (∼12) after 30 days, followed by MC (∼10), both significantly higher than FL. Of 16 tested ions, 9 were cumulatively released in quantifiable amounts. FL showed the highest F⁻ (279 µg/mL) and Sr²⁺ (128 µg/mL) release. LL released the most Cl⁻ (101 µg/mL) and PO₄³ ⁻ (31 µg/mL), while SO₄²⁻ peaked in TC (111 µg/mL). DY released the most Ca²⁺ (950 µg/mL). EXP showed the highest NH₄⁺ levels, MC the highest K⁺, and LL the highest Na⁺.

Significance: The differences in ion release and pH values represent a mosaic stone in understanding the biomineralization effects of liner materials, particularly when considered alongside monomer release data in future studies addressing biological and clinical contexts.

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离子色谱法研究生物活性牙衬材料的离子释放。

目的：应用生物活性牙衬材料促进牙本质桥的形成，减少早期牙髓炎症，支持牙髓再矿化。离子释放是理解它们在生物矿化过程中作用的关键因素。本研究首次采用单一方法定量和定性分析了多种衬里材料中多种离子的同时释放。方法：采用离子色谱法测定16种阴离子和阳离子在第1、4、7、14、30天随pH变化释放到水中的情况。测试的衬垫材料包括来自Solventum （EXP）、TheraCal LC （TC）、Dycal （DY）、Lime-Lite Enhanced （LL）、Fuji Lining LC Paste Pak （FL）和Medcem MTA （MC）的实验衬垫/基底。统计分析采用Shapiro-Wilk、Kruskal-Wallis、friedman检验（α = 0.05）。结果：30天后，DY的pH值最高（~ 12），其次是MC(~ 10)，两者均显著高于FL。在所测的16个离子中，有9个以可量化的量累积释放。FL的释放量最高（279 µg/mL）， Sr 2⁺的释放量最高（128 µg/mL）。LL释放最多的是Cl（101 µg/mL）和PO₄³ （31 µg/mL），而SO₄²在TC（111 µg/mL）中释放最多。DY释放的Ca 2 +最多（950 µg/mL）。EXP中NH₄+含量最高，MC中K +含量最高，LL中Na +含量最高。意义：离子释放和pH值的差异代表了理解内衬材料生物矿化效应的马赛克石，特别是当在未来的生物学和临床研究中考虑单体释放数据时。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Dental Materials 工程技术-材料科学：生物材料

CiteScore

9.80

自引率

10.00%

发文量

290

审稿时长

67 days

期刊介绍： Dental Materials publishes original research, review articles, and short communications. Academy of Dental Materials members click here to register for free access to Dental Materials online. The principal aim of Dental Materials is to promote rapid communication of scientific information between academia, industry, and the dental practitioner. Original Manuscripts on clinical and laboratory research of basic and applied character which focus on the properties or performance of dental materials or the reaction of host tissues to materials are given priority publication. Other acceptable topics include application technology in clinical dentistry and dental laboratory technology. Comprehensive reviews and editorial commentaries on pertinent subjects will be considered.