Mechanistic insights into enhanced volatile pyridine biodegradation through shortcut pyridine-N transformation in counter-diffusion biofilms by regulating biofilm stratification and microbial spatial interactions

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology Pub Date : 2025-07-17 DOI:10.1016/j.biortech.2025.133010

Peng Zheng , Yan Li , Youpeng Cheng , Jing Wang , Yixuan Wang , Yang Mu , Jinyou Shen

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Abstract

Air pollution and nitrogen contamination residues remain challenges in the conventional biological treatment of industrial wastewater containing volatile organic compounds. In this study, counter-diffusion biofilms were integrated with shortcut nitrification–denitrification technology to enhance pyridine biodegradation through shortcut pyridine-N transformation (EPB-SPNT), with emphasis on biofilm stratification and microbial spatial interactions. Results showed that the removal efficiency of pyridine and total nitrogen reached 100 % and 91.24 ± 0.75 %, respectively. Fluorescence in situ hybridization and metagenomic analysis revealed that aerobic pyridine-degrading bacteria (APDB, Alicycliphilus) and ammonia-oxidizing bacteria (AOB, Nitrosomonas) were located in the aerobic layer, while anoxic pyridine degrading-denitrifying bacteria (APD-DB, Paracoccus) were enriched in the anoxic layer. Biofilm stability was mainly attributed to the lower hydrophilicity of protein secondary structure. The EPB-SPNT process was driven by the spatial cooperation among APDB, AOB, and APD-DB. These findings demonstrate the feasibility of implementing the EPB-SPNT in counter-diffusion biofilms through the regulation of microbial stratification and interactions.

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通过调节生物膜分层和微生物空间相互作用，通过反扩散生物膜中的短程吡啶- n转化增强挥发性吡啶生物降解的机理

空气污染和氮污染残留物是传统生物处理含挥发性有机物工业废水的难题。本研究将反扩散生物膜与短程硝化-反硝化技术相结合，通过短程吡啶- n转化（EPB-SPNT）增强吡啶的生物降解，重点关注生物膜分层和微生物空间相互作用。结果表明，该工艺对吡啶和总氮的去除率分别为100 %和91.24 ± 0.75 %。荧光原位杂交和元基因组分析显示，好氧吡啶降解菌（APDB, aliicycliphilus）和氨氧化菌（AOB, Nitrosomonas）主要分布在好氧层，而缺氧吡啶降解反硝化菌（APD-DB, Paracoccus）主要富集在缺氧层。生物膜的稳定性主要归因于蛋白质二级结构的亲水性较低。EPB-SPNT过程是由APDB、AOB和APD-DB之间的空间合作驱动的。这些发现证明了EPB-SPNT通过调控微生物分层和相互作用在反扩散生物膜中实现的可行性。

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来源期刊

Bioresource Technology 工程技术-能源与燃料

CiteScore

20.80

自引率

19.30%

发文量

2013

审稿时长

12 days

期刊介绍： Bioresource Technology publishes original articles, review articles, case studies, and short communications covering the fundamentals, applications, and management of bioresource technology. The journal seeks to advance and disseminate knowledge across various areas related to biomass, biological waste treatment, bioenergy, biotransformations, bioresource systems analysis, and associated conversion or production technologies. Topics include: • Biofuels: liquid and gaseous biofuels production, modeling and economics • Bioprocesses and bioproducts: biocatalysis and fermentations • Biomass and feedstocks utilization: bioconversion of agro-industrial residues • Environmental protection: biological waste treatment • Thermochemical conversion of biomass: combustion, pyrolysis, gasification, catalysis.