Simultaneous bio-clogging mitigation and nutrient removal enhancement via sulfide addition in constructed wetlands

IF 13.3 1区工程技术 Q1 ENGINEERING, CHEMICAL

Chemical Engineering Journal Pub Date : 2025-07-19 DOI:10.1016/j.cej.2025.166194

Jie Wang, Yueying Zheng, Xiaodi Zhou, Xiaoning Wang, Yabing Lv, Juchen Xu, Ruiya Chen, Xugang He, Jie Hou

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Abstract

Bio-clogging, primarily driven by excessive biofilm growth, significantly hampers the efficiency and longevity of constructed wetlands (CWs). This study introduces an innovative sulfide addition strategy to mitigate bio-clogging and enhance nitrogen and phosphorus removal in CWs. Experimental results indicate sulfide addition notably slows bio-clogging by up to 3.72-fold, primarily through targeted disruption of extracellular polymeric substances, particularly polysaccharides. Scanning electron microscopy confirmed that biofilms in sulfide-treated CWs developed porous, network-like structures, significantly improving substrate permeability. Three-dimensional fluorescence excitation-emission matrix analyses revealed substantial production of tryptophan-like proteins and microbial byproducts under anaerobic conditions in sulfide-treated CWs, which were subsequently fermented into short-chain fatty acids. Under carbon-limited conditions (COD/N = 3), sulfide-enhanced CWs achieved approximately 78.19 % and 84.23 % removal efficiencies for nitrogen and phosphorus, respectively—roughly 20 % higher than conventional CWs. Mechanistically, enhanced nitrogen and phosphorus removal in SCW primarily resulted from short-cut nitrification, increased carbon source availability promoting denitrification, sulfur-driven autotrophic denitrification and sulfur related denitrifying dephosphatation. Overall, these findings propose sulfide addition as a viable approach for sustainable CW operation.

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在人工湿地中添加硫化物可同时缓解生物堵塞和增强营养物去除

生物堵塞主要是由过度的生物膜生长引起的，严重影响了人工湿地的效率和寿命。本研究介绍了一种创新的硫化物添加策略，以减轻生物堵塞，提高化粪池中氮和磷的去除。实验结果表明，硫化物的加入显著减缓了高达3.72倍的生物堵塞，主要是通过有针对性地破坏细胞外聚合物物质，特别是多糖。扫描电镜证实，硫化物处理过的化化水中的生物膜形成了多孔的网状结构，显著提高了基质的渗透性。三维荧光激发-发射矩阵分析显示，硫化物处理后的CWs在厌氧条件下大量产生色氨酸样蛋白和微生物副产物，随后发酵成短链脂肪酸。在碳限条件下（COD/N = 3），硫化物增强化粪池对氮和磷的去除率分别约为78.19 %和84.23 %，比常规化粪池高约20 %。从机理上讲，SCW中氮磷去除的增强主要源于短程硝化、碳源可用性的增加促进反硝化、硫驱动的自养反硝化和硫相关的反硝化脱磷。总的来说，这些发现表明硫化物添加是可持续连续油管操作的可行方法。

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来源期刊

Chemical Engineering Journal 工程技术-工程：化工

CiteScore

21.70

自引率

9.30%

发文量

6781

审稿时长

2.4 months

期刊介绍： The Chemical Engineering Journal is an international research journal that invites contributions of original and novel fundamental research. It aims to provide an international platform for presenting original fundamental research, interpretative reviews, and discussions on new developments in chemical engineering. The journal welcomes papers that describe novel theory and its practical application, as well as those that demonstrate the transfer of techniques from other disciplines. It also welcomes reports on carefully conducted experimental work that is soundly interpreted. The main focus of the journal is on original and rigorous research results that have broad significance. The Catalysis section within the Chemical Engineering Journal focuses specifically on Experimental and Theoretical studies in the fields of heterogeneous catalysis, molecular catalysis, and biocatalysis. These studies have industrial impact on various sectors such as chemicals, energy, materials, foods, healthcare, and environmental protection.