A red fluorescent probe for lipid droplet polarity sensitivity and identification of nonalcoholic fatty liver disease

IF 4.2 3区工程技术 Q2 CHEMISTRY, APPLIED

Dyes and Pigments Pub Date : 2025-07-17 DOI:10.1016/j.dyepig.2025.113048

Kun Yu , Jie Wang , Yuqing Wang , Jing Yang , Chunfei Wang , Lei Hu , Hui Wang

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引用次数: 0

Abstract

The increasing prevalence of non-alcoholic fatty liver disease (NAFLD) has attracted widespread attention in recent years. As critical intracellular sites for energy storage, lipid droplets (LDs) have become one of the important biomarkers for early diagnosis of NAFLD. Therefore, a series of fluorescent probes (WL1-WL3) with D-π-A-D and D-π-A structures were designed and synthesized in this study. Photophysical property studies shown that all these probes are highly sensitive to polarity and insensitive to various analytes and pH changes. Among them, probe WL3 showed excellent optical properties, with an emission wavelength in the near-infrared region (>650 nm), a large Stokes shift, and a good linear correlation between fluorescence intensity and solvent polarity. Further biological results demonstrated that probe WL3 not only monitors alterations in the number and size of LDs, but also detects variations in the polarity of intracellular LDs and dynamically tracks LDs-related processes (LDs autophagy). In a NAFLD cell model, probe WL3 could effectively monitor the efficacy of metformin (Met) in the treatment of NAFLD. Furthermore, probe WL3 was able to clearly distinguish normal liver from NAFLD at both the tissue and organ level, demonstrating its promising potential for clinical potential.

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一种用于脂滴极性敏感性和非酒精性脂肪肝鉴别的红色荧光探针

近年来，非酒精性脂肪性肝病（NAFLD）的日益流行引起了人们的广泛关注。脂滴作为细胞内能量储存的关键部位，已成为NAFLD早期诊断的重要生物标志物之一。因此，本研究设计并合成了一系列具有D-π-A-D和D-π-A结构的荧光探针（WL1-WL3）。光物理性质研究表明，这些探针对极性高度敏感，对各种分析物和pH变化不敏感。其中，探针WL3表现出优异的光学性能，其发射波长在近红外区域（>650 nm）， Stokes位移大，荧光强度与溶剂极性之间具有良好的线性相关性。进一步的生物学结果表明，探针WL3不仅可以监测lld数量和大小的变化，还可以检测细胞内lld极性的变化，并动态跟踪lld相关过程（lld自噬）。在NAFLD细胞模型中，探针WL3可以有效监测二甲双胍（Met）治疗NAFLD的疗效。此外，探针WL3能够在组织和器官水平上清晰地区分正常肝脏和NAFLD，显示了其良好的临床应用潜力。

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来源期刊

Dyes and Pigments 工程技术-材料科学：纺织

CiteScore

8.20

自引率

13.30%

发文量

933

审稿时长

33 days

期刊介绍： Dyes and Pigments covers the scientific and technical aspects of the chemistry and physics of dyes, pigments and their intermediates. Emphasis is placed on the properties of the colouring matters themselves rather than on their applications or the system in which they may be applied. Thus the journal accepts research and review papers on the synthesis of dyes, pigments and intermediates, their physical or chemical properties, e.g. spectroscopic, surface, solution or solid state characteristics, the physical aspects of their preparation, e.g. precipitation, nucleation and growth, crystal formation, liquid crystalline characteristics, their photochemical, ecological or biological properties and the relationship between colour and chemical constitution. However, papers are considered which deal with the more fundamental aspects of colourant application and of the interactions of colourants with substrates or media. The journal will interest a wide variety of workers in a range of disciplines whose work involves dyes, pigments and their intermediates, and provides a platform for investigators with common interests but diverse fields of activity such as cosmetics, reprographics, dye and pigment synthesis, medical research, polymers, etc.