A simple and low-cost method for fluoride analysis of plant materials using alkali extraction and ion-selective electrode.

IF 4.4 2区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

Plant Methods Pub Date : 2025-07-17 DOI:10.1186/s13007-025-01412-6

Chenyu Zhang, Mark G M Aarts, Antony van der Ent

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引用次数: 0

Abstract

Backgrounds: Existing methods for fluoride (F^-) determination in plant material require expensive equipment and specialized reagents. This study aimed to develop a simple and cost-effective method for fluoride analysis in plant samples.

Results: Using an orthogonal assay design with certified reference material, this study optimized a sodium hydroxide extraction method (5 mol·L^-1) with heating at 120 °C for 0.5 h, followed by the addition of potassium acetate, ionic strength adjustment, and measurement via an ion-selective electrode. The method achieved a limit of detection (LOD) and limit of quantification (LOQ) of 1.41 and 4.71 mg·kg⁻¹, respectively. Recovery rates ranged from 84.74 to 89.34% in Arabidopsis thaliana (intraday relative standard deviation [RSD] ≤ 2.31%, inter-day RSD ≤ 4.17%) and from 83.53 to 91.55% in Camellia sinensis (intraday RSD ≤ 3.11%, inter-day RSD ≤ 4.98%). In A. thaliana cultivated in NaF-dosed (500 µM) nutrient solution, the fluoride concentration in the shoot was 16.00 mg·kg^-1; In C. sinensis grown under 250 µM NaF treatment, the shoot fluoride concentration was 292.71 mg·kg^-1. Moreover, the fluoride concentration in Tea products purchased from local supermarkets ranged from 16.28 to 61.78 mg kg^-1.

Conclusion: This study presents a simple, reliable, and cost-effective method for fluoride analysis in plant materials, which can be further validated through inter-laboratory testing to establish a standardized approach.

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查看原文本刊更多论文

碱萃取和离子选择电极分析植物材料中氟化物的简单、低成本方法。

背景：现有的植物材料中氟化物（F-）测定方法需要昂贵的设备和专用试剂。本研究旨在建立一种简单、经济的植物样品氟分析方法。结果：采用标准物质正交试验设计，优化了氢氧化钠（5 mol·L-1）提取方法，120℃加热0.5 h，加入乙酸钾，调节离子强度，离子选择电极测定。该方法的检出限和定量限分别为1.41和4.71 mg·kg⁻（毒毒学）。拟南芥的回收率为84.74 ~ 89.34%（日内相对标准偏差[RSD]≤2.31%，日内RSD≤4.17%），山茶的回收率为83.53 ~ 91.55%（日内RSD≤3.11%，日内RSD≤4.98%）。在添加naf（500µM）的营养液中培养的拟南蓝，茎部氟浓度为16.00 mg·kg-1；在250µM NaF处理下生长的中华香椿，其茎部氟浓度为292.71 mg·kg-1。此外，从本地超市购买的茶叶产品的氟化物浓度介乎16.28至61.78毫克公斤-1。结论：本研究提出了一种简单、可靠、经济的植物材料氟分析方法，可通过实验室间测试进一步验证，建立标准化方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Methods 生物-植物科学

CiteScore

9.20

自引率

3.90%

发文量

121

审稿时长

2 months

期刊介绍： Plant Methods is an open access, peer-reviewed, online journal for the plant research community that encompasses all aspects of technological innovation in the plant sciences. There is no doubt that we have entered an exciting new era in plant biology. The completion of the Arabidopsis genome sequence, and the rapid progress being made in other plant genomics projects are providing unparalleled opportunities for progress in all areas of plant science. Nevertheless, enormous challenges lie ahead if we are to understand the function of every gene in the genome, and how the individual parts work together to make the whole organism. Achieving these goals will require an unprecedented collaborative effort, combining high-throughput, system-wide technologies with more focused approaches that integrate traditional disciplines such as cell biology, biochemistry and molecular genetics. Technological innovation is probably the most important catalyst for progress in any scientific discipline. Plant Methods’ goal is to stimulate the development and adoption of new and improved techniques and research tools and, where appropriate, to promote consistency of methodologies for better integration of data from different laboratories.