C-glycosylated and alkylated flavonoids with potent anti-inflammatory activity from the whole plants of Drymaria cordata

IF 3.4 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Phytochemistry Pub Date : 2025-07-16 DOI:10.1016/j.phytochem.2025.114624

Lan-Yi Lu , Yan-Hua Wu , Peng Zhang , Li-Yan Wang

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Abstract

Drymaria cordata is a traditional Chinese medicinal plant used for the treatment of hepatitis and chronic nephritis. Six previously undescribed flavonoid C-glycosides (1–3, 8, 9, 11), two unreported alkylated flavonoids (4, 5), and four known flavonoids (6, 7, 10, 12) were isolated from its 70 % ethanol extract. The structures were established by extensive analysis of NMR, HRMS, and X-ray single-crystal diffraction. All compounds exhibited inhibitory activity against nitric oxide production, with compound 8, which contains a rare sugar moiety (3,5-dihydroxy-6-methyl-γ-pyrone), showing the most pronounced effect. Further investigation revealed that 8 suppressed the expression of several key cytokines and inhibited the phosphorylation of p65. Molecular docking studies indicated that 8 targeted IKK, an upstream regulator of the NF-κB pathway, thereby blocking its activation.

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具有抗炎活性的c -糖基化和烷基化黄酮类化合物。

血莲是一种传统的中药植物，用于治疗肝炎和慢性肾炎。从其70%乙醇提取物中分离出6个先前未描述的类黄酮c -糖苷（1- 3,8,9,11），2个未报道的烷基化类黄酮（4,5）和4个已知的类黄酮（6,7,10,12）。通过NMR， HRMS和x射线单晶衍射的广泛分析确定了其结构。所有化合物都表现出抑制一氧化氮生成的活性，其中化合物8的抑制作用最明显，因为化合物8含有一个罕见的糖片段（3,5-二羟基-6-甲基-γ-pyrone）。进一步研究发现，8抑制了几种关键细胞因子的表达，抑制了p65的磷酸化。分子对接研究表明，8靶向NF-κB通路上游调控因子IKK，从而阻断其激活。

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来源期刊

Phytochemistry 生物-植物科学

CiteScore

6.40

自引率

7.90%

发文量

443

审稿时长

39 days

期刊介绍： Phytochemistry is a leading international journal publishing studies of plant chemistry, biochemistry, molecular biology and genetics, structure and bioactivities of phytochemicals, including ''-omics'' and bioinformatics/computational biology approaches. Phytochemistry is a primary source for papers dealing with phytochemicals, especially reports concerning their biosynthesis, regulation, and biological properties both in planta and as bioactive principles. Articles are published online as soon as possible as Articles-in-Press and in 12 volumes per year. Occasional topic-focussed special issues are published composed of papers from invited authors.