Review of research progress in immobilization and chemical modification of microbial enzymes and their application.

IF 4.3 2区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Walaa A Abdel Wahab
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引用次数: 0

Abstract

Enzyme stabilization is one of the most critical steps help in applying the enzymes on industrial scale efficiently. Enzymes have use in a variety of areas, including medical, industry, food, and even beauty and cosmetics. The industrial application of enzymes is constantly limited by stability and cost. Enzyme synthesis on a large scale involves multiple steps, therefore stability and repeatability are critical. These two goals are crucial on an industrial scale because they translate to reduced time, effort, and cost. Enzyme stabilization provides the stability and reusability required for successful application. Immobilization with appropriate carriers and conjugation with chemically modified polysaccharides are the most common and low-cost strategies used for enzyme stabilization. These tactics enhance the enzyme's physicochemical characteristics, making it better suited for industrial applications that benefit our daily lives. This review is an attempt to provide a spot on each method, their progress, benefits and draw backs, and their application.

微生物酶的固定化、化学修饰及其应用研究进展综述。
酶稳定是酶在工业生产中有效应用的关键步骤之一。酶在许多领域都有用途,包括医疗、工业、食品,甚至美容和化妆品。酶的工业应用一直受到稳定性和成本的限制。大规模的酶合成涉及多个步骤,因此稳定性和可重复性至关重要。这两个目标在工业规模上是至关重要的,因为它们可以减少时间、精力和成本。酶稳定提供了成功应用所需的稳定性和可重用性。用合适的载体固定和用化学修饰的多糖偶联是最常用的和低成本的酶稳定策略。这些策略增强了酶的物理化学特性,使其更适合于有益于我们日常生活的工业应用。这篇综述试图对每种方法、它们的进展、优点和缺点以及它们的应用进行介绍。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Microbial Cell Factories
Microbial Cell Factories 工程技术-生物工程与应用微生物
CiteScore
9.30
自引率
4.70%
发文量
235
审稿时长
2.3 months
期刊介绍: Microbial Cell Factories is an open access peer-reviewed journal that covers any topic related to the development, use and investigation of microbial cells as producers of recombinant proteins and natural products, or as catalyzers of biological transformations of industrial interest. Microbial Cell Factories is the world leading, primary research journal fully focusing on Applied Microbiology. The journal is divided into the following editorial sections: -Metabolic engineering -Synthetic biology -Whole-cell biocatalysis -Microbial regulations -Recombinant protein production/bioprocessing -Production of natural compounds -Systems biology of cell factories -Microbial production processes -Cell-free systems
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