Potential involvement of IL-40 in kidney disease associated to systemic lupus erythematosus.

IF 1.9 4区 医学 Q3 RHEUMATOLOGY
Lupus Pub Date : 2025-10-01 Epub Date: 2025-07-18 DOI:10.1177/09612033251361037
Chiara Rizzo, Lidia La Barbera, Marianna Lo Pizzo, Federica Camarda, Leila Mohammadnezhad, Francesco Dieli, Francesco Ciccia, Serena Fasano, Giuliana Guggino
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引用次数: 0

Abstract

ObjectivesAim of this study was to investigate the expression of interleukin (IL)-40, a cytokine associated with B cells homoeostasis and immune response, in Systemic lupus erythematosus (SLE) and SLE associated nephritis.Methods32 patients with SLE and 21 controls were enrolled. Serum concentration of IL-40 was assessed by ELISA and cellular sources of IL-40 were determined by flow-cytometry. An in vitro assay with recombinant IL-40 (rIL-40) was performed to detect the effect on cytokine production from peripheral blood mononuclear cells (PBMC). In kidney biopsies obtained from patients with lupus nephritis (LN) immunofluorescence was performed to assess IL-40 expression and detect its cellular sources at tissue level.ResultsSerum IL-40 levels show a decreasing trend in SLE with LN, compared with SLE without LN and controls. No differences between SLE and controls were evidenced in urinary IL-40 levels; in the percentage of CD3+IL40+, CD19+IL40+ and CD14+IL40+; and in the production of pro-inflammatory cytokines by PBMC stimulated with rIL-40. IL-40 expression was markedly increased in kidney tissue from LN patients, with a significant increase of IL40+ cells in LN samples compared to controls, as confirmed by the quantification analysis.ConclusionTo the best of our knowledge this is the first demonstration of IL-40 expression at kidney level in SLE. These preliminary data suggest a possible role of IL-40 in LN. In particular, IL-40 could be a marker of kidney tissue damage, although its specific mechanism of action needs to be further elucidated.

IL-40在系统性红斑狼疮相关肾脏疾病中的潜在参与
目的研究白细胞介素(IL)-40在系统性红斑狼疮(SLE)和SLE相关性肾炎中的表达,IL -40是一种与B细胞稳态和免疫反应相关的细胞因子。方法32例SLE患者和21例对照组。ELISA法检测血清IL-40浓度,流式细胞术检测IL-40细胞来源。用重组白细胞介素40 (IL-40)体外检测其对外周血单核细胞(PBMC)细胞因子产生的影响。在狼疮性肾炎(LN)患者的肾脏活检中,采用免疫荧光法评估IL-40的表达并在组织水平上检测其细胞来源。结果合并LN的SLE患者血清IL-40水平与不合并LN的SLE患者及对照组相比呈下降趋势。SLE患者与对照组尿IL-40水平无差异;CD3+IL40+、CD19+IL40+和CD14+IL40+的百分比;并通过il -40刺激PBMC产生促炎细胞因子。定量分析证实,LN患者肾组织中IL-40表达明显增加,LN样本中IL-40 +细胞与对照组相比显著增加。结论:据我们所知,这是首次在SLE中证实IL-40在肾脏水平表达。这些初步数据提示IL-40在LN中的可能作用。特别是,IL-40可能是肾脏组织损伤的标志物,尽管其具体的作用机制有待进一步阐明。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Lupus
Lupus 医学-风湿病学
CiteScore
4.20
自引率
11.50%
发文量
225
审稿时长
1 months
期刊介绍: The only fully peer reviewed international journal devoted exclusively to lupus (and related disease) research. Lupus includes the most promising new clinical and laboratory-based studies from leading specialists in all lupus-related disciplines. Invaluable reading, with extended coverage, lupus-related disciplines include: Rheumatology, Dermatology, Immunology, Obstetrics, Psychiatry and Cardiovascular Research…
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