The impact of neddylation on prognosis in the immune microenvironment of neuroblastoma: a single-cell transcriptomic analysis.

IF 3.3 4区 医学 Q3 IMMUNOLOGY
Juan You, Hu Yang
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引用次数: 0

Abstract

Neuroblastoma (NB) is a prevalent malignant tumor in children, primarily affecting the nervous system, with a high mortality rate, particularly in high-risk patients. This study aims to establish a neddylation-related prognostic signature (NRPS) through the analysis of gene expression data from NB patients and to evaluate its clinical application potential. A comprehensive analysis was conducted using gene expression profiling, single-cell RNA sequencing (scRNA-seq), weighted gene co-expression network analysis (WGCNA), and single-sample gene set enrichment analysis (ssGSEA) to elucidate the role of neddylation and establish a NRPS for NB patients. Our analysis identified 171,992 cells categorized into 19 distinct clusters, revealing that Schwann and tumor cells exhibited significantly higher neddylation scores compared to other cell types. We constructed a NRPS that significantly impacts overall survival and event-free survival. Immune infiltration analysis demonstrated significantly higher levels of immune cells, particularly activated CD8 + T cells and natural killer cells, in the low-risk group, indicating a stronger anti-tumor immune response. Furthermore, gene set enrichment analysis (GSEA) revealed that the high-risk group was associated with cell division and DNA repair pathways, while the low-risk group showed enrichment in immune-related signaling pathways, suggesting immune activation may confer protective effects. Additionally, the immunotherapy analysis suggested that the low-risk score group exhibited better immunotherapy outcomes. This study underscores the clinical significance of NRPS in predicting survival and therapeutic outcomes in NB patients, highlighting their necessity and urgency in personalized treatment approaches.

类化修饰对神经母细胞瘤免疫微环境预后的影响:单细胞转录组学分析。
神经母细胞瘤(Neuroblastoma, NB)是儿童常见的恶性肿瘤,主要影响神经系统,死亡率高,特别是在高危患者中。本研究旨在通过对NB患者基因表达数据的分析,建立类化修饰相关预后特征(NRPS),并评估其临床应用潜力。通过基因表达谱、单细胞RNA测序(scRNA-seq)、加权基因共表达网络分析(WGCNA)、单样本基因集富集分析(ssGSEA)等综合分析,阐明类化修饰的作用,建立NB患者NRPS。我们的分析确定了171,992个细胞分为19个不同的簇,揭示了与其他细胞类型相比,雪旺细胞和肿瘤细胞表现出显着更高的类化修饰评分。我们构建了一个显著影响总生存率和无事件生存率的NRPS。免疫浸润分析显示,低风险组的免疫细胞水平明显更高,特别是活化的CD8 + T细胞和自然杀伤细胞,表明抗肿瘤免疫反应更强。此外,基因集富集分析(GSEA)显示,高风险组与细胞分裂和DNA修复途径相关,而低风险组在免疫相关信号通路中富集,提示免疫激活可能具有保护作用。此外,免疫治疗分析表明,低风险评分组表现出更好的免疫治疗结果。本研究强调了NRPS在预测NB患者生存和治疗结果方面的临床意义,强调了其在个性化治疗方法中的必要性和紧迫性。
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来源期刊
Immunologic Research
Immunologic Research 医学-免疫学
CiteScore
6.90
自引率
0.00%
发文量
83
审稿时长
6-12 weeks
期刊介绍: IMMUNOLOGIC RESEARCH represents a unique medium for the presentation, interpretation, and clarification of complex scientific data. Information is presented in the form of interpretive synthesis reviews, original research articles, symposia, editorials, and theoretical essays. The scope of coverage extends to cellular immunology, immunogenetics, molecular and structural immunology, immunoregulation and autoimmunity, immunopathology, tumor immunology, host defense and microbial immunity, including viral immunology, immunohematology, mucosal immunity, complement, transplantation immunology, clinical immunology, neuroimmunology, immunoendocrinology, immunotoxicology, translational immunology, and history of immunology.
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