Pancreatic injury induces β-cell regeneration in axolotl.

IF 1.5 3区 生物学 Q2 ANATOMY & MORPHOLOGY
Connor J Powell, Hani D Singer, Ashley R Juarez, Ryan T Kim, Elane Kim, Duygu Payzin-Dogru, Aaron M Savage, Noah J Lopez, Kara Thornton, Steven J Blair, Adnan Abouelela, Anita Dittrich, Stuart G Akeson, Miten Jain, Jessica L Whited
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Abstract

Background: Diabetes is a condition characterized by a loss of pancreatic β-cell function, which results in the dysregulation of insulin homeostasis. Using a partial pancreatectomy model in axolotl, we aimed to observe the pancreatic response to injury.

Results: Here we show a comprehensive histological characterization of pancreatic islets in axolotl. Following pancreatic injury, no apparent blastema-like structure was observed. We found a significant, organ-wide increase in cellular proliferation post-resection in the pancreas compared to sham-operated controls. This proliferative response was most robust at the site of injury. Further, an increase in nuclear density was observed, suggesting compensatory congestion as a mechanism of regeneration. We found that β-cells actively contributed to the increased rates of proliferation upon injury. β-Cell proliferation manifested in increased β-cell mass in injured tissue at 2 weeks post-injury. At 4 weeks post-injury, we found organ-wide proliferation to be extinguished while proliferation at the injury site persisted, corresponding to pancreatic tissue recovery. Similarly, total β-cell mass was comparable to sham after 4 weeks.

Conclusions: Our findings suggest a non-blastema-mediated regeneration process takes place in the pancreas, by which pancreatic resection induces whole-organ β-cell proliferation without the formation of a blastemal structure. This process is analogous to other models of compensatory congestion in axolotl.

胰腺损伤诱导蝾螈β细胞再生。
背景:糖尿病是一种以胰腺β细胞功能丧失为特征的疾病,导致胰岛素稳态失调。采用蝾螈部分胰腺切除术模型,观察胰腺对损伤的反应。结果:在这里,我们展示了一种全面的蝾螈胰岛组织学特征。胰腺损伤后未见明显囊胚样结构。我们发现,与假手术对照组相比,胰腺切除术后细胞增殖明显增加。这种增殖反应在损伤部位最为强烈。此外,观察到核密度的增加,表明代偿性充血是再生的机制。我们发现β-细胞在损伤后积极促进增殖速率的增加。损伤后2周,β细胞增殖表现为损伤组织中β细胞质量增加。在损伤后4周,我们发现全器官的增殖消失,而损伤部位的增殖持续存在,对应于胰腺组织的恢复。同样,4周后β细胞总质量与假手术相当。结论:我们的研究结果表明,胰腺发生了非胚母介导的再生过程,胰腺切除术诱导了全器官β细胞增殖,而没有形成胚母结构。这一过程类似于美西螈的其他代偿性充血模型。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Developmental Dynamics
Developmental Dynamics 生物-发育生物学
CiteScore
5.10
自引率
8.00%
发文量
116
审稿时长
3-8 weeks
期刊介绍: Developmental Dynamics, is an official publication of the American Association for Anatomy. This peer reviewed journal provides an international forum for publishing novel discoveries, using any model system, that advances our understanding of development, morphology, form and function, evolution, disease, stem cells, repair and regeneration.
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