Association of α-SMA/FAP-positive cancer-associated fibroblasts with clinicopathological features and prognosis in extrahepatic cholangiocarcinoma.

IF 2.3 3区 医学 Q2 PATHOLOGY
Xiaojun Sui, Zhangqiang Zheng, Lei Yang, Dapeng Zhang, Xiangyu Sun, Ximo Wang
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引用次数: 0

Abstract

Objective: This study aimed to evaluate the value of cancer-associated fibroblasts (CAFs) that positively express α-smooth muscle actin (α-SMA) and fibroblast activation protein (FAP) as survival indicators for patients with extrahepatic cholangiocarcinoma (eCCA).

Methods: The clinicopathological data of eCCA patients who underwent surgical treatment in Tianjin Nankai Hospital from January 1, 2019, to December 31, 2022, were retrospectively analysed. A total of 79 patients were included, 49 were male and 30 were female, with an age of (64.3 ± 8.3) years. Clinicopathological data such as age, gender, tumour location, lymph node metastasis, tumour differentiation degree, and TNM stage of the patients were recorded. The expressions of α-SMA and FAP, the markers of CAFs, in eCCA were detected by immunohistochemistry. The relationships between the expressions of the two proteins and the clinicopathological data and prognosis of the patients were analysed.

Results: The positive expressions of α-SMA and FAP in CAFs were observed in 78.5% (62/79) and 35.4% (28/79) of the patients, respectively. There was a highly positive correlation between the expression of α-SMA and that of FAP (r = 0.992, P < 0.001). Univariate analysis showed that CAFs with positive FAP expression and tumour location were statistically significant in terms of overall survival time and recurrence-free survival time. Multivariate analysis indicated that positive FAP expression and tumour location might be independent factors affecting overall survival time and recurrence-free survival time.

Conclusions: CAFs with positive FAP expression may be a prognostic indicator of poor postoperative survival in eCCA patients and may serve as an independent predictor of poor postoperative survival rate in these patients.

Abstract Image

Abstract Image

α-SMA/ fap阳性癌相关成纤维细胞与肝外胆管癌临床病理特征及预后的关系
目的:本研究旨在评价阳性表达α-平滑肌肌动蛋白(α-SMA)和成纤维细胞活化蛋白(FAP)的癌相关成纤维细胞(CAFs)作为肝外胆管癌(eCCA)患者生存指标的价值。方法:回顾性分析2019年1月1日至2022年12月31日在天津市南开医院行手术治疗的eCCA患者的临床病理资料。共纳入79例患者,其中男性49例,女性30例,年龄(64.3±8.3)岁。记录患者的年龄、性别、肿瘤位置、淋巴结转移、肿瘤分化程度、TNM分期等临床病理资料。免疫组织化学法检测cas标志物α-SMA和FAP在eCCA中的表达。分析两种蛋白的表达与患者临床病理资料及预后的关系。结果:78.5%(62/79)患者CAFs中α-SMA和FAP表达阳性,35.4%(28/79)患者CAFs中α-SMA和FAP表达阳性。α-SMA与FAP的表达呈高度正相关(r = 0.992, P)。结论:FAP阳性表达的cas可能是eCCA患者术后生存不良的预后指标,可作为该患者术后生存不良的独立预测指标。
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来源期刊
Diagnostic Pathology
Diagnostic Pathology 医学-病理学
CiteScore
4.60
自引率
0.00%
发文量
93
审稿时长
1 months
期刊介绍: Diagnostic Pathology is an open access, peer-reviewed, online journal that considers research in surgical and clinical pathology, immunology, and biology, with a special focus on cutting-edge approaches in diagnostic pathology and tissue-based therapy. The journal covers all aspects of surgical pathology, including classic diagnostic pathology, prognosis-related diagnosis (tumor stages, prognosis markers, such as MIB-percentage, hormone receptors, etc.), and therapy-related findings. The journal also focuses on the technological aspects of pathology, including molecular biology techniques, morphometry aspects (stereology, DNA analysis, syntactic structure analysis), communication aspects (telecommunication, virtual microscopy, virtual pathology institutions, etc.), and electronic education and quality assurance (for example interactive publication, on-line references with automated updating, etc.).
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