Effect of Intermittent Hypoxia on the Expression of Glucocorticoid Receptors and Hypoxia-Inducible Factor in Rat Brain.

IF 0.6 4区医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL

Bulletin of Experimental Biology and Medicine Pub Date : 2025-05-01 Epub Date: 2025-07-18 DOI:10.1007/s10517-025-06436-5

E A Rybnikova, K A Baranova, M Y Zenko

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Abstract

The effect of intermittent normobaric hypoxia on the expression of hypoxia-inducible factor 1α (HIF-1α) and glucocorticoid receptors in the brain of male Wistar rats was studied in the context of developing neuroprotective methods of hypoxytherapy. Hypoxia in three modes of different effectiveness was modeled by three cycles of 5-min inhalation of a hypoxic mixture (9, 12, or 16% O₂) with 15-min intervals of normoxia (21% O₂) over 3 days. Immunopositive staining for glucocorticoid receptors and HIF-1α in rat brain increased, which correlated with the protective effectiveness of the regimen. The protective mode (9% O₂) increased the expression of these proteins in all regions, the medium mode (12% O₂) stimulated expression of glucocorticoid receptors only in layer V of the neocortex and HIF-1α only in the CA1 field. The least effective mode (16% O₂) did not affect these parameters. Thus, neuroprotective normobaric hypoxia activates the same adaptive mechanisms as hypobaric hypoxia.

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间歇性缺氧对大鼠脑糖皮质激素受体及缺氧诱导因子表达的影响。

研究了间歇性正压缺氧对雄性Wistar大鼠脑内缺氧诱导因子1α (HIF-1α)和糖皮质激素受体表达的影响。三种不同效果模式下的缺氧通过3个循环，分别吸入5分钟的低氧混合物（9、12或16% O2），并在3天内间隔15分钟的常氧（21% O2）来模拟。大鼠脑糖皮质激素受体和HIF-1α免疫阳性染色增加，这与方案的保护效果相关。保护模式（9% O2）增加了这些蛋白在所有区域的表达，培养基模式（12% O2）只刺激了新皮层V层糖皮质激素受体的表达，只刺激了CA1区HIF-1α的表达。最低有效模式（16% O2）对这些参数没有影响。因此，神经保护性常压缺氧激活了与低压缺氧相同的适应机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bulletin of Experimental Biology and Medicine 医学-医学：研究与实验

CiteScore

1.50

自引率

14.30%

发文量

265

审稿时长

2 months

期刊介绍： Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005. World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells. The Russian Volume Year is published in English from April.