Microbial degradation of textile dye reactive blue 250 (RB 250) by the novel Pseudomonas aeruginosa RGB11: a sustainable approach.

IF 1.9 4区 生物学 Q3 MICROBIOLOGY
Brazilian Journal of Microbiology Pub Date : 2025-09-01 Epub Date: 2025-07-18 DOI:10.1007/s42770-025-01743-5
Garima Chetry, Vasantha Veerappa Lakshmaiah
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引用次数: 0

Abstract

Untreated textile effluent discharge has increased due to rapid industrialization and human activity, posing a serious and growing threat to environmental well-being over the past few decades. This study isolates a novel bacterial strain, Pseudomonas aeruginosa RGB11, from sewage sludge capable of degrading the azo dye Reactive Blue 250 dye (RB 250) as identified via 16S rRNA gene sequencing. Decolorization of RB 250 was tested in Minimal Salt Media (MSM) throughout the studies. Under static conditions, pH 7, and 37 °C, the dye showed 88.91% decolorization after 24 h, which increased to 96.27% at pH 9 and 93.15% at 45 °C. Adding 1% w/v sucrose and yeast extract as carbon and nitrogen sources increased the decolorization to 96.83% and 97.54%, respectively. A study on growth kinetics over 8 h showed that as the Optical Density (OD) of bacteria increased at 600 nm, the absorbance of the dye decreased at 604 nm, indicating the bacterial role in the decolorization process. FT-IR analysis of the metabolite extracted after decolorization revealed the shift in the intensity of the characteristic peak of the RB 250 dye and the formation of new peaks, which can be attributed to the degradation of dye and generation of intermediates in the decolorized solution. The LC-MS study further confirms degradation as the parental peak in the dye disappeared and smaller new peaks were observed, possibly due to breakage of characteristic bonds in dye like the azo bond. Haemolysis test on blood agar demonstrated gamma haemolysis confirming that the novel strain of Pseudomonas aeruginosa RGB11 doesn't produce haemolysins and is a non-pathogenic strain. Thus, emphasizing the efficiency of Pseudomonas aeruginosa RGB11 as potential candidate for dye decolourizer for textile effluents. It has immense potential to serve as a contributor to environmental studies by providing a means of sustainable bioremediation for textile effluents.

新型铜绿假单胞菌RGB11对纺织染料活性蓝250的微生物降解研究
在过去的几十年里,由于快速的工业化和人类活动,未经处理的纺织废水排放量不断增加,对环境健康构成了严重且日益严重的威胁。本研究从污水污泥中分离出一株新型细菌——铜绿假单胞菌RGB11,该菌株通过16S rRNA基因测序鉴定出能够降解偶氮染料活性蓝250染料(RB 250)。在整个研究过程中,在最低盐培养基(MSM)中测试了RB 250的脱色效果。在静态条件下,pH为7,37℃,24 h脱色率为88.91%,pH为9时脱色率为96.27%,45℃时脱色率为93.15%。添加1% w/v的蔗糖和酵母浸膏作为碳源和氮源,脱色率分别达到96.83%和97.54%。8 h的生长动力学研究表明,随着细菌在600 nm处光密度(Optical Density, OD)的增加,染料在604 nm处的吸光度降低,表明细菌在脱色过程中起作用。对脱色后提取的代谢物进行FT-IR分析,发现RB 250染料特征峰的强度发生了变化,并形成了新的峰,这可能是由于脱色溶液中染料的降解和中间体的生成。LC-MS研究进一步证实了降解,染料中的亲本峰消失,观察到较小的新峰,可能是由于染料中偶氮键等特征键的断裂。血液琼脂溶血试验显示γ溶血,证实该新型铜绿假单胞菌RGB11不产生溶血素,为非致病性菌株。因此,强调铜绿假单胞菌RGB11作为纺织废水染料脱色剂的潜力。它具有巨大的潜力,可以为环境研究作出贡献,为纺织废水提供可持续的生物修复手段。
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来源期刊
Brazilian Journal of Microbiology
Brazilian Journal of Microbiology 生物-微生物学
CiteScore
4.10
自引率
4.50%
发文量
216
审稿时长
1.0 months
期刊介绍: The Brazilian Journal of Microbiology is an international peer reviewed journal that covers a wide-range of research on fundamental and applied aspects of microbiology. The journal considers for publication original research articles, short communications, reviews, and letters to the editor, that may be submitted to the following sections: Biotechnology and Industrial Microbiology, Food Microbiology, Bacterial and Fungal Pathogenesis, Clinical Microbiology, Environmental Microbiology, Veterinary Microbiology, Fungal and Bacterial Physiology, Bacterial, Fungal and Virus Molecular Biology, Education in Microbiology. For more details on each section, please check out the instructions for authors. The journal is the official publication of the Brazilian Society of Microbiology and currently publishes 4 issues per year.
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