Odontogenic/osteogenic differentiation of dental pulp stem cells on a Biodentine-coated polymer nanofibers.

IF 2.9 4区 医学 Q3 ENGINEERING, BIOMEDICAL
Zahra Sarvarian, Parisa Sanaei-Rad, Farzad Moradikhah, Ehsan Seyedjafari, Mohammad Javanbakht
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引用次数: 0

Abstract

Background: Tissue engineering has become increasingly applied for tissue repair purposes. Scaffolds, one of the main components of tissue engineering, provide a supportive framework for cell culture and growth. The objective of the present study was to investigate the odontogenic/osteogenic differentiation of dental pulp stem cells, cultured on a polycaprolactone (PCL)-based nanofibrous scaffold, coated with Biodentine. This study evaluated the use of Biodentine as a coating on nanofiber scaffolds and investigated the biological effects of this material on the differentiation of dental pulp stem cells, which hold promising applications in dental and bone tissue engineering.

Methods: This study is a basic research investigation. Initially, PCL nanofibrous scaffolds were produced through electrospinning, followed by a post-fabrication surface modification step. The morphology and properties of the scaffolds were examined using scanning electron microscopy (SEM). In the surface treatment step, two different concentrations of Biodentine (0.05% and 0.01%) were applied on the mats. The biocompatibility of the scaffolds was assessed using an MTT assay on days 1, 3, and 5. Additionally, the odontogenic/osteogenic differentiation potency of fabricated scaffolds was evaluated by alkaline phosphatase (ALP) activity and deposited calcium of the cells on days 7, 14, and 21.

Results: SEM analysis revealed that Biodentine coating increased surface roughness, particularly at the 0.05% concentration, where excessive particle aggregation was observed. In contrast, the control PCL scaffold exhibited a well-organized fibrous structure with a smooth surface, whereas the 0.01% Biodentine-coated scaffold displayed a moderately roughened surface with uniformly distributed mineralized deposits. Cell viability was higher in the 0.01% Biodentine group, while the 0.05% concentration showed reduced proliferation. ALP activity peaked on day 14, and the highest level of calcium deposition was observed in the 0.01% Biodentine group on day 21, indicating enhanced biomineralization.

Conclusion: Biodentine/PCL scaffolds demonstrated notable and suitable physical and chemical properties. Furthermore, they enhanced odontogenic/osteogenic differentiation and mineralization compared to the control group. These findings support the potential of fabricated scaffolds for odontogenic/osteogenic differentiation applications.

生物牙本质素包覆聚合物纳米纤维对牙髓干细胞成牙/成骨分化的影响。
背景:组织工程在组织修复方面的应用越来越广泛。支架是组织工程的主要组成部分之一,为细胞培养和生长提供了支持框架。本研究的目的是研究牙髓干细胞在聚己内酯(PCL)基纳米纤维支架上培养的成牙性/成骨性分化。本研究评估了生物牙本质作为纳米纤维支架涂层的应用,并研究了该材料对牙髓干细胞分化的生物学效应,该材料在口腔和骨组织工程中具有广阔的应用前景。方法:本研究为基础研究调查。最初,PCL纳米纤维支架是通过静电纺丝生产的,然后是加工后的表面改性步骤。利用扫描电子显微镜(SEM)对支架的形貌和性能进行了观察。在表面处理步骤中,将0.05%和0.01%两种不同浓度的生物登定涂在草席上。在第1、3、5天采用MTT法评估支架的生物相容性。此外,在第7、14和21天,通过碱性磷酸酶(ALP)活性和细胞沉积钙来评估制备的支架的成牙/成骨分化能力。结果:扫描电镜分析显示,生物登汀涂层增加了表面粗糙度,特别是在0.05%浓度下,观察到过度的颗粒聚集。相比之下,对照PCL支架呈现出组织良好的纤维结构和光滑的表面,而0.01%生物牙登汀涂层支架则呈现出适度粗糙的表面和均匀分布的矿化沉积物。0.01% Biodentine组细胞活力较高,0.05% Biodentine组细胞增殖能力降低。ALP活性在第14天达到峰值,0.01%奥登汀组钙沉积在第21天达到最高水平,表明生物矿化增强。结论:生物牙汀/PCL支架具有良好的理化性能。此外,与对照组相比,它们增强了牙源性/成骨性分化和矿化。这些发现支持了合成支架在牙源性/成骨分化应用中的潜力。
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来源期刊
BioMedical Engineering OnLine
BioMedical Engineering OnLine 工程技术-工程:生物医学
CiteScore
6.70
自引率
2.60%
发文量
79
审稿时长
1 months
期刊介绍: BioMedical Engineering OnLine is an open access, peer-reviewed journal that is dedicated to publishing research in all areas of biomedical engineering. BioMedical Engineering OnLine is aimed at readers and authors throughout the world, with an interest in using tools of the physical and data sciences and techniques in engineering to understand and solve problems in the biological and medical sciences. Topical areas include, but are not limited to: Bioinformatics- Bioinstrumentation- Biomechanics- Biomedical Devices & Instrumentation- Biomedical Signal Processing- Healthcare Information Systems- Human Dynamics- Neural Engineering- Rehabilitation Engineering- Biomaterials- Biomedical Imaging & Image Processing- BioMEMS and On-Chip Devices- Bio-Micro/Nano Technologies- Biomolecular Engineering- Biosensors- Cardiovascular Systems Engineering- Cellular Engineering- Clinical Engineering- Computational Biology- Drug Delivery Technologies- Modeling Methodologies- Nanomaterials and Nanotechnology in Biomedicine- Respiratory Systems Engineering- Robotics in Medicine- Systems and Synthetic Biology- Systems Biology- Telemedicine/Smartphone Applications in Medicine- Therapeutic Systems, Devices and Technologies- Tissue Engineering
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