Dentinal remineralisation using self-assembling peptide P11-4 and chloro calcium phosphosilicate in obese children: An ex vivo study

Abstract

Objective

Saliva acts as primary oral defence agent. Obese children’s saliva differs in composition from healthy children. The objective of the study was to evaluate the remineralization effects in saliva from obese versus healthy children by using P11–4 and CCPS.

Design

After gel induced demineralisation, 20 P11–4 and 20 CCPS treated dentinal discs were submerged in natural whole saliva collected from healthy and obese children (n = 10). Micro-CT and Scanning Electron Microscopy (SEM) analyses were performed at baseline, 7-days (T1 stage), and 28-days (T2 stage).

Results

CCPS had enhanced mineral content percentage and significantly greater efficacy in lowering lesion depth. Group 2 displayed the most efficacy at 68.8 %, while Group 3 showed the lowest efficacy. P11–4 and CCPS demonstrated diminished efficacy when exposed to saliva from obese children compared to healthy ones. P11–4 exhibited a more significant reduction in lesion depth at the T1 stage; however, its effectiveness decreased with extended saliva exposure at T2 stage. The percentage of remineralisation efficacy was calculated after normalising the data against sound dentine values. Two-way ANOVA indicated significant differences across the groups exclusively at stage T2 (p < 0.005). SEM micrographs demonstrated significant mineral deposition and tubule obstruction. There were substantial disparities across groups, with CCPS controls demonstrating the most efficient tubule sealing. The P11–4 group exhibited the minimal tubular occlusion.

Conclusion

CCPS in healthy children saliva after 28-days had enhanced remineralisation efficacy compared to P11–4.