Evaluation of the optimal phytosphingosine pretreatment time for enhancing the resin dentin interface

Abstract

This study comparatively evaluates the effect of dentin pretreatment with 6.5 % proanthocyanidin and 6.5 % phytosphingosine at two different time intervals using micro-shear bond strength and contact angle analyses. A total of 120 coronal dentin samples was sectioned and subjected to artificial cariogenic solution for 2 weeks. 60 samples were further divided based on the pretreatment agents (6.5 % PAC and 6.5 % PHS) into 2 groups (n = 24) and control (n = 12). Based on the pretreatment application time, the samples were further subdivided into subgroup A – 30s, B – 60s. Micro shear bond strength was analysed using universal testing machine and contact angle was evaluated using goniometer. The mode of failure was determined under a stereomicroscope. Data was tabulated and statistically analysed using Welch's ANOVA and Multiple comparison of groups was done using Games Howell test. Pretreatment using PHS on demineralized dentin at 30s showed the highest shear bond strength followed by PAC and control group. At 30s and 60s, PHS and PAC when compared to the control group showed a highly significant difference in-terms of micro-shear bond strength and contact angle analysis (p < 0.05). Therefore, PHS shows an improved bond strength of carious dentin to the resin interface and hence can be considered as a promising alternative collagen crosslinking agent to PAC.