Óscar Rapado-González , Cristina Isabel Sevilla-García , Juan Pizcueta-Leirós , Ángel Salgado-Barreira , María Piñeiro-Lamas , Félix De Carlos-Villafranca , Rafael López-López , María Mercedes Suárez-Cunqueiro
{"title":"ASSOCIATION OF DNA METHYLATION AND ORAL CANCER RISK: A SYSTEMATIC REVIEW AND META-ANALYSIS","authors":"Óscar Rapado-González , Cristina Isabel Sevilla-García , Juan Pizcueta-Leirós , Ángel Salgado-Barreira , María Piñeiro-Lamas , Félix De Carlos-Villafranca , Rafael López-López , María Mercedes Suárez-Cunqueiro","doi":"10.1016/j.jebdp.2025.102169","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>DNA promoter methylation is one of the main epigenetic mechanisms of silencing of tumor-suppressor genes in cancer. Accumulating scientific evidence has shown various genes with aberrant DNA methylation in oral cancer (OC), however, the magnitude of the association between DNA methylation and OC risk remains controversial.</div></div><div><h3>Objective</h3><div>To evaluate the overall and specific impact of DNA promoter methylation on the risk of OC development.</div></div><div><h3>Material and methods</h3><div>PubMed, EMBASE, Web of Science, and the Cochrane Library were searched for eligible studies. The Newcastle-Ottawa Scale (NOS) was used to evaluate the methodological quality of the included studies with a case-control design. The pooled odds ratios (ORs) and 95% confidence intervals (CIs) were calculated to estimate the strength of the associations with R software; and Egger’s test was used to detect publication bias.</div></div><div><h3>Results</h3><div>A total of 41 studies including 4218 OC patients and 3478 noncancer controls were included in the meta-analysis. Overall, a significant association was found between DNA promoter methylation and OC risk (OR = 5.83, <em>95% CI</em> 4.14-8.20; <em>P</em> < .001). In addition, the pooled ORs showed a significant association between specific tumor-related genes and OC risk: <em>p16</em> (5.77; <em>95% CI</em> 3.95-8.45; <em>P</em> < .001), <em>ECAD</em> (4.47; <em>95% CI</em> 2.77-7.21; <em>P</em> < .001), <em>MGMT</em> (3.85; <em>95% CI</em> 2.48-5.97; <em>P</em> < .001), <em>DAPK</em> (5.58; <em>95% CI</em> 2.14-14.56; <em>P</em> < .001), <em>hMLH1</em> (10.48; <em>95% CI</em> 1.04-106.1; <em>P = .047</em>), <em>p14</em> (3.21; <em>95% CI</em> 1.78-5.78; <em>P</em> < .001), and <em>p15</em> (5.02; <em>95% CI</em> 2.76-9.12; <em>P</em> < .001).</div></div><div><h3>Conclusion</h3><div>A significant overall association was found between gene promoter methylation and OC risk. Specifically, the promoter methylation of <em>p16, ECAD, MGMT, DAPK, hMLH1, p15,</em> and <em>p14</em> displayed a significant role in oral carcinogenesis, acting as promising biomarkers for OC prediction and prognosis.</div></div>","PeriodicalId":48736,"journal":{"name":"Journal of Evidence-Based Dental Practice","volume":"25 4","pages":"Article 102169"},"PeriodicalIF":4.1000,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Evidence-Based Dental Practice","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1532338225000843","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
引用次数: 0
Abstract
Background
DNA promoter methylation is one of the main epigenetic mechanisms of silencing of tumor-suppressor genes in cancer. Accumulating scientific evidence has shown various genes with aberrant DNA methylation in oral cancer (OC), however, the magnitude of the association between DNA methylation and OC risk remains controversial.
Objective
To evaluate the overall and specific impact of DNA promoter methylation on the risk of OC development.
Material and methods
PubMed, EMBASE, Web of Science, and the Cochrane Library were searched for eligible studies. The Newcastle-Ottawa Scale (NOS) was used to evaluate the methodological quality of the included studies with a case-control design. The pooled odds ratios (ORs) and 95% confidence intervals (CIs) were calculated to estimate the strength of the associations with R software; and Egger’s test was used to detect publication bias.
Results
A total of 41 studies including 4218 OC patients and 3478 noncancer controls were included in the meta-analysis. Overall, a significant association was found between DNA promoter methylation and OC risk (OR = 5.83, 95% CI 4.14-8.20; P < .001). In addition, the pooled ORs showed a significant association between specific tumor-related genes and OC risk: p16 (5.77; 95% CI 3.95-8.45; P < .001), ECAD (4.47; 95% CI 2.77-7.21; P < .001), MGMT (3.85; 95% CI 2.48-5.97; P < .001), DAPK (5.58; 95% CI 2.14-14.56; P < .001), hMLH1 (10.48; 95% CI 1.04-106.1; P = .047), p14 (3.21; 95% CI 1.78-5.78; P < .001), and p15 (5.02; 95% CI 2.76-9.12; P < .001).
Conclusion
A significant overall association was found between gene promoter methylation and OC risk. Specifically, the promoter methylation of p16, ECAD, MGMT, DAPK, hMLH1, p15, and p14 displayed a significant role in oral carcinogenesis, acting as promising biomarkers for OC prediction and prognosis.
期刊介绍:
The Journal of Evidence-Based Dental Practice presents timely original articles, as well as reviews of articles on the results and outcomes of clinical procedures and treatment. The Journal advocates the use or rejection of a procedure based on solid, clinical evidence found in literature. The Journal''s dynamic operating principles are explicitness in process and objectives, publication of the highest-quality reviews and original articles, and an emphasis on objectivity.