Metal ion–mediated dual–polysaccharide coatings enhanced the microenvironmental homeostasis and adhesion capacity of Lactiplantibacillus plantarum

IF 11 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids Pub Date : 2025-07-14 DOI:10.1016/j.foodhyd.2025.111761

Jingyu Si, Yujin Li, Changhu Xue, Zihao Wei

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引用次数: 0

Abstract

The instability of probiotics during processing and gastrointestinal delivery severely limited their large-scale applications. Inspired by dual defense systems in nature, this study engineered a metal ion–mediated dual–polysaccharide coating system through interfacial assembly of sodium alginate and guar gum, achieving simultaneous enhancement of microenvironmental homeostasis and adhesion capacity of Lactiplantibacillus plantarum. Multidimensional characterization techniques verified the uniform deposition of the composite coating on bacterial surfaces and its favorable biocompatibility. Spectral analysis revealed that characteristic functional groups of the coatings formed hierarchically assembled networks with bacterial surface glycoproteins via multiple supramolecular interactions. Additionally, the coating exhibited precise adaptation to bacterial surface structures, significantly enhancing bacteria–coating interfacial binding strength and mucosal adhesion efficiency through molecular anchoring strategies. Crucially, the highly dense structure and polymer clustering effects within the composite coating established dual defense barriers, ensuring microenvironmental homeostasis under complex biointerfacial stresses (e.g., strong acidic environments, thermo–mechanical stress and UV irradiation). This study introduced a novel delivery strategy, establishing the foundation for the long-term survival and functional transformation of probiotics.

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金属离子介导的双多糖包被增强了植物乳杆菌的微环境稳态和粘附能力

益生菌在加工和胃肠道传递过程中的不稳定性严重限制了其大规模应用。本研究受自然界双防御系统的启发，通过海藻酸钠和瓜尔胶的界面组装，设计了一种金属离子介导的双多糖包被系统，同时增强了植物乳杆菌的微环境稳态和粘附能力。多维表征技术验证了复合涂层在细菌表面的均匀沉积和良好的生物相容性。光谱分析表明，涂层的特征官能团通过多种超分子相互作用与细菌表面糖蛋白形成分层组装网络。此外，该涂层对细菌表面结构具有精确的适应性，通过分子锚定策略显著提高了细菌-涂层的界面结合强度和粘膜粘附效率。至关重要的是，复合涂层内的高密度结构和聚合物聚集效应建立了双重防御屏障，确保了在复杂的生物界面应力（如强酸环境、热机械应力和紫外线照射）下的微环境稳态。本研究提出了一种新的给药策略，为益生菌的长期存活和功能转化奠定了基础。

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来源期刊

Food Hydrocolloids 工程技术-食品科技

CiteScore

19.90

自引率

14.00%

发文量

871

审稿时长

37 days

期刊介绍： Food Hydrocolloids publishes original and innovative research focused on the characterization, functional properties, and applications of hydrocolloid materials used in food products. These hydrocolloids, defined as polysaccharides and proteins of commercial importance, are added to control aspects such as texture, stability, rheology, and sensory properties. The research's primary emphasis should be on the hydrocolloids themselves, with thorough descriptions of their source, nature, and physicochemical characteristics. Manuscripts are expected to clearly outline specific aims and objectives, include a fundamental discussion of research findings at the molecular level, and address the significance of the results. Studies on hydrocolloids in complex formulations should concentrate on their overall properties and mechanisms of action, while simple formulation development studies may not be considered for publication. The main areas of interest are: -Chemical and physicochemical characterisation Thermal properties including glass transitions and conformational changes- Rheological properties including viscosity, viscoelastic properties and gelation behaviour- The influence on organoleptic properties- Interfacial properties including stabilisation of dispersions, emulsions and foams- Film forming properties with application to edible films and active packaging- Encapsulation and controlled release of active compounds- The influence on health including their role as dietary fibre- Manipulation of hydrocolloid structure and functionality through chemical, biochemical and physical processes- New hydrocolloids and hydrocolloid sources of commercial potential. The Journal also publishes Review articles that provide an overview of the latest developments in topics of specific interest to researchers in this field of activity.