Surface organization of aptamers via diazonium grafting: A key parameter in label-free electrochemical sensing

IF 4.2 3区工程技术 Q2 ELECTROCHEMISTRY

Electrochemistry Communications Pub Date : 2025-07-16 DOI:10.1016/j.elecom.2025.108000

Teodora Lupoi , Bogdan Feier , Florence Geneste , Cecilia Cristea , Yann R. Leroux

{"title":"Surface organization of aptamers via diazonium grafting: A key parameter in label-free electrochemical sensing","authors":"Teodora Lupoi , Bogdan Feier , Florence Geneste , Cecilia Cristea , Yann R. Leroux","doi":"10.1016/j.elecom.2025.108000","DOIUrl":null,"url":null,"abstract":"<div><div>The spatial arrangement of biorecognition molecules on the sensor surface plays a critical role in determining the performance of electrochemical biosensors. In this work, we report a covalent and tunable immobilization strategy using aryl diazonium chemistry to functionalize carbon electrodes with ethynyl groups protected by trimethylsilyl (TMS) or triisopropylsilyl (TIPS) moieties. After deprotection, an azide-modified aptamer (APT) specific to diclofenac (DCF) was immobilized via copper-catalyzed azide–alkyne cycloaddition (CuAAC). Although the TMS and TIPS groups differ in size by only 1.7 Å, this small variation significantly influenced APT spacing and sensor performance. The TIPS-based sensor displayed a nearly fourfold increase in signal response compared to the TMS-based counterpart, achieving a limit of detection of 17.95 μM. These results underscore the importance of nanoscale molecular design in optimizing label-free aptasensor sensitivity.</div></div>","PeriodicalId":304,"journal":{"name":"Electrochemistry Communications","volume":"178 ","pages":"Article 108000"},"PeriodicalIF":4.2000,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Electrochemistry Communications","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1388248125001390","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ELECTROCHEMISTRY","Score":null,"Total":0}

引用次数: 0

Abstract

The spatial arrangement of biorecognition molecules on the sensor surface plays a critical role in determining the performance of electrochemical biosensors. In this work, we report a covalent and tunable immobilization strategy using aryl diazonium chemistry to functionalize carbon electrodes with ethynyl groups protected by trimethylsilyl (TMS) or triisopropylsilyl (TIPS) moieties. After deprotection, an azide-modified aptamer (APT) specific to diclofenac (DCF) was immobilized via copper-catalyzed azide–alkyne cycloaddition (CuAAC). Although the TMS and TIPS groups differ in size by only 1.7 Å, this small variation significantly influenced APT spacing and sensor performance. The TIPS-based sensor displayed a nearly fourfold increase in signal response compared to the TMS-based counterpart, achieving a limit of detection of 17.95 μM. These results underscore the importance of nanoscale molecular design in optimizing label-free aptasensor sensitivity.

查看原文本刊更多论文

通过重氮接枝的适体表面组织：无标记电化学传感的关键参数

生物识别分子在传感器表面的空间排列对电化学生物传感器的性能起着至关重要的作用。在这项工作中，我们报告了一种共价和可调的固定策略，使用芳基重氮化学来功能化碳电极，其乙基由三甲基硅基（TMS）或三异丙基硅基（TIPS）保护。脱保护后，通过铜催化叠氮化物-炔环加成（CuAAC）固定化双氯芬酸（DCF）特异性叠氮化物修饰适配体（APT）。虽然TMS组和TIPS组的大小仅相差1.7 Å，但这一微小的差异显著影响了APT间距和传感器性能。与基于tms的传感器相比，基于tips的传感器的信号响应增加了近四倍，达到了17.95 μM的检测极限。这些结果强调了纳米级分子设计在优化无标记适体传感器灵敏度方面的重要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Electrochemistry Communications 工程技术-电化学

CiteScore

8.50

自引率

3.70%

发文量

160

审稿时长

1.2 months

期刊介绍： Electrochemistry Communications is an open access journal providing fast dissemination of short communications, full communications and mini reviews covering the whole field of electrochemistry which merit urgent publication. Short communications are limited to a maximum of 20,000 characters (including spaces) while full communications and mini reviews are limited to 25,000 characters (including spaces). Supplementary information is permitted for full communications and mini reviews but not for short communications. We aim to be the fastest journal in electrochemistry for these types of papers.