Signatures of tumor microenvironment-related genes and long noncoding RNAs predict poor prognosis in osteosarcoma.

IF 2.6 3区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

PLoS ONE Pub Date : 2025-07-16 eCollection Date: 2025-01-01 DOI:10.1371/journal.pone.0326876

Ying Lu, Li Zhou, Zhanyu Yang

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Abstract

Introduction: Osteosarcoma is an aggressive bone cancer with poor outcomes, especially in young individuals. This study sought to identify tumor microenvironment-related genes (TMIEGs) and associated long noncoding RNAs (TMIELs) that influence patient prognosis.

Materials and methods: Data from the TARGET osteosarcoma and GTEx muscle datasets were analysed to calculate stromal and immune scores, dividing patients into high- and low-score groups. Differential gene expression was assessed, and prognostic TMIELs and TMIEGs were identified through regression analyses. Prognostic signatures were evaluated via Kaplan‒Meier curves, receiver operating characteristic (ROC) analysis, and Cox regression, while immune cell composition was analysed via CIBERSORT.

Results: Three prognostic TMIELs (AC090559.1, LINC01549, SENCR) and three TMIEGs (DOK2, RHBDL2, NPW) were identified. High-risk patients have poorer survival outcomes, with immune processes possibly reducing the risk of osteosarcoma. Prognostic signatures effectively predict overall survival.

Conclusion: TMIEGs and TMIELs can reliably predict survival in patients with osteosarcoma, suggesting their potential as therapeutic biomarkers and the need for further research.

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肿瘤微环境相关基因和长链非编码rna的特征预测骨肉瘤的不良预后。

骨肉瘤是一种预后不良的侵袭性骨癌，尤其是在年轻人中。本研究旨在鉴定影响患者预后的肿瘤微环境相关基因（TMIEGs）和相关长链非编码rna （TMIELs）。材料和方法：分析TARGET骨肉瘤和GTEx肌肉数据集的数据，计算基质和免疫评分，将患者分为高分组和低分组。评估差异基因表达，并通过回归分析确定预后TMIELs和tmigs。预后特征通过Kaplan-Meier曲线、受试者工作特征（ROC）分析和Cox回归进行评估，免疫细胞组成通过CIBERSORT进行分析。结果：鉴定出3个预后TMIELs （AC090559.1、LINC01549、SENCR）和3个tmigs （DOK2、RHBDL2、NPW）。高风险患者的生存结果较差，免疫过程可能降低骨肉瘤的风险。预后特征可有效预测总生存期。结论：tmigs和TMIELs可以可靠地预测骨肉瘤患者的生存，提示其作为治疗性生物标志物的潜力，需要进一步研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

PLoS ONE 生物-生物学

CiteScore

6.20

自引率

5.40%

发文量

14242

审稿时长

3.7 months

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