Antimicrobial potential and biological properties of modified glass-ionomer cement supplemented with chlorhexidine diacetate.

IF 1.7 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of oral science Pub Date : 2025-01-01 DOI:10.2334/josnusd.25-0068

Suwanna Jitpukdeebodintra, Sissada Tannukit, Nattapon Rotpenpian

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引用次数: 0

Abstract

Purpose: This study aimed to investigate the minimum inhibitory concentration (MIC) and biological properties of modified glass-ionomer cement (BIO-GIC) supplemented with chlorhexidine diacetate (CHX).

Methods: The BIO-GIC was originally a combining formulation of conventional glass-ionomer cement, chitosan, albumin and tricalcium phosphate. CHX was incorporated at various concentrations of 1.25%, 2%, 2.5%, 5%, 7.5% and 10%. Antimicrobial activity against certain microorganisms related to cariogenesis Streptococcus mutans (S. mutans), Streptococcus sanguinis (S. sanguinis), and Enterococcus faecalis (E. faecalis), was evaluated by microdilution test. One effective concentration of CHX was mixed with Penaeus merguiensis-Translationally Controlled Tumor Protein (Pmer-TCTP) and further conducted by biological tests including cell viability and mineralization in dental pulp.

Results: S. mutans were inhibited at 7.5% CHX-modified BIO-GIC, while S. sanguinis and E. faecalis showed no inhibition at any concentration. Cell viability significantly decreased with 7.5% CHX-modified BIO-GIC after 72 h. Dental pulp exposed to this group formed smaller and fewer calcium nodules but adding Pmer-TCTP reduced cell death and slightly enhanced mineralization.

Conclusion: 7.5% CHX-modified BIO-GIC shows potential for inhibiting S. mutans and having potential cell viability and mineralization in dental pulp cells.

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添加双醋酸氯己定改性玻璃离子水泥的抗菌潜力和生物学性能。

目的：研究添加双醋酸氯己定（CHX）的改性玻璃离子水泥（BIO-GIC）的最低抑菌浓度（MIC）和生物学性能。方法：BIO-GIC最初是由常规玻璃离子水泥、壳聚糖、白蛋白和磷酸三钙组合而成。CHX掺入浓度分别为1.25%、2%、2.5%、5%、7.5%和10%。用微量稀释试验评价了对某些与龋齿发生有关的变形链球菌（S. mutans）、血链球菌（S. sanguinis）和粪肠球菌（E. faecalis）的抑菌活性。将一个有效浓度的CHX与merguiensis对虾-翻译控制肿瘤蛋白（Pmer-TCTP）混合，进一步进行牙髓细胞活力和矿化等生物学试验。结果：7.5% chx修饰的BIO-GIC对变形链球菌有抑制作用，而血链球菌和粪肠球菌在任何浓度下均无抑制作用。添加7.5% chx修饰的BIO-GIC后72 h，细胞活力显著降低。暴露于该组牙髓的钙结节更小、更少，但添加Pmer-TCTP可减少细胞死亡，并略微增强矿化。结论：7.5% chx修饰的BIO-GIC具有抑制变形链球菌的潜力，具有潜在的牙髓细胞活力和矿化能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of oral science DENTISTRY, ORAL SURGERY & MEDICINE-MATERIALS SCIENCE, BIOMATERIALS

CiteScore

3.80

自引率

0.00%

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