Comparative Satellitomics in Arowanas (Telostei, Osteoglossiformes) Sheds Light on the Evolution of Ancient Satellite DNAs.

IF 3.7 1区 生物学 Q1 ZOOLOGY
Fernando Henrique Santos de Souza, Gustavo Akira Toma, Jhon Alex Dziechciarz Vidal, Manuel Angel Garrido-Ramos, Guilherme Mota Souza, Rodrigo Zeni Dos Santos, Fábio Porto-Foresti, Thomas Liehr, Ricardo Utsunomia, Marcelo de Bello Cioffi
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引用次数: 0

Abstract

A significant fraction of the genomes of most multicellular eukaryotes includes extensive arrays of tandemly repeated sequences, collectively referred to as satellite DNAs (satDNAs). However, the mechanisms responsible for generating and maintaining varying satDNA abundances across lineages and temporal scales are still unclear. This work focused on arowana fishes (Teleostei, Osteoglossiformes) as a model; their widespread intercontinental distribution and basal phylogenetic position within Teleostei make them a compelling model for evolutionary research, especially in the realm of satDNA molecular evolution. Through the integration of genomic and chromosomal data, we analyzed and compared the catalogs of satDNA families (i.e., satellitomes) of four out of the six extant arowana species, elucidating ancestral evolutionary trends and establishing their temporal history. Arowanas displayed a small number of satDNA families, ranging from 16 to 25 in Osteoglossum bicirrhosum and Scleropages formosus, respectively. Alongside the identification of some shared satDNAs, many considered species-specific, nonetheless possess a limited number of copies in other species. The minimal variation observed both within and across species highlights the long-term conservation of satDNAs during evolution, since specific ones (referred to as long-term conserved satDNAs) may have endured throughout a lengthy evolutionary period. Moreover, fluorescence in situ hybridization (FISH) investigations conducted with the most abundant satDNAs demonstrated unique hybridization patterns for homologous orthologous ones, signifying their dynamic genomic positioning. Besides, the similarities of satDNAs among species align with their phylogenetic relationships, showing the high dynamism of arowanas' satDNAs, with several evolutionary events driving their sequence diversity.

龙鱼(远骨目,骨舌形目)的比较卫星组学揭示了古代卫星dna的进化。
大多数多细胞真核生物的基因组中有很大一部分包括大量串联重复序列,统称为卫星dna (satdna)。然而,负责产生和维持不同谱系和时间尺度的不同satDNA丰度的机制仍然不清楚。本研究以龙鱼(Teleostei, Osteoglossiformes)为模型;它们广泛的洲际分布和在Teleostei中的基础系统发育位置使它们成为进化研究的一个引人注目的模型,特别是在卫星dna分子进化领域。通过整合基因组和染色体数据,对现存6种龙鱼中4种的卫星dna家族(即卫星组)目录进行了分析和比较,阐明了其祖先进化趋势,并建立了其时间历史。龙鱼有少量的satDNA家族,分别在biirrhosum Osteoglossum和formosus Scleropages中有16 ~ 25个。除了确定一些共有的satdna外,许多被认为是物种特异性的,尽管如此,在其他物种中拥有有限数量的拷贝。在物种内部和物种间观察到的最小变异突出了进化过程中satdna的长期保存,因为特定的satdna(称为长期保存的satdna)可能在漫长的进化过程中持续存在。此外,对最丰富的satdna进行的荧光原位杂交(FISH)研究显示,同源同源dna的杂交模式独特,表明它们的动态基因组定位。此外,物种间satdna的相似性与其系统发育关系一致,表明了龙鱼satdna的高度动态性,若干进化事件推动了其序列多样性。
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来源期刊
CiteScore
6.40
自引率
12.10%
发文量
81
审稿时长
>12 weeks
期刊介绍: The official journal of the International Society of Zoological Sciences focuses on zoology as an integrative discipline encompassing all aspects of animal life. It presents a broader perspective of many levels of zoological inquiry, both spatial and temporal, and encourages cooperation between zoology and other disciplines including, but not limited to, physics, computer science, social science, ethics, teaching, paleontology, molecular biology, physiology, behavior, ecology and the built environment. It also looks at the animal-human interaction through exploring animal-plant interactions, microbe/pathogen effects and global changes on the environment and human society. Integrative topics of greatest interest to INZ include: (1) Animals & climate change (2) Animals & pollution (3) Animals & infectious diseases (4) Animals & biological invasions (5) Animal-plant interactions (6) Zoogeography & paleontology (7) Neurons, genes & behavior (8) Molecular ecology & evolution (9) Physiological adaptations
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