Mechanism of miR-107/HMOX1 axis in hepatic sinusoidal endothelial cells stimulated by ischemia-reperfusion injury.

IF 2.7 3区生物学

Hereditas Pub Date : 2025-07-16 DOI:10.1186/s41065-025-00495-4

Zhao Wang, Jiangyang Sun, Yu Wang, Yichuan Zhang, Laian Ge

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引用次数: 0

Abstract

Background: Liver ischemia-reperfusion injury (IRI) is a common complication of diseases such as liver transplantation, hepatic resection, and hemorrhagic shock. This study aimed to elucidate the molecular mechanism of miR-107 affecting hepatic ischemia-reperfusion injury (IRI).

Methods: The expression changes of miR-107 during hepatic IRI were quantified using quantitative real-time PCR. Subsequently, in vitro cellular experiments were carried out to verify the role of miR-107 on hypoxia/reoxygenation (HR) through CCK-8, flow cytometer, and commercial kits. In terms of mechanism, it was determined that miR-107 had a regulatory relationship with target genes through luciferase reporter assay.

Results: In mouse liver IRI, miR-107 expression was increased while HMOX1 expression was decreased in liver tissues. In vitro cellular experiments, miR-107 inhibitors favored the alleviation of proliferation, apoptosis, inflammation, and oxidative stress in HR-damaged liver sinusoidal endothelial cells. In the molecular mechanism study, we determined that miR-107 could bind to HMOX1 and inhibit the HMOX1 expression. Low HMOX1 expression could eliminate the protective effect of miR-107 inhibitors.

Conclusion: MiR-107 expression was elevated during hepatic IRI and exacerbates hepatic injury by targeting HMOX1 inhibition.

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缺血-再灌注损伤刺激肝窦内皮细胞miR-107/HMOX1轴表达的机制

背景：肝缺血再灌注损伤（IRI）是肝移植、肝切除术和失血性休克等疾病的常见并发症。本研究旨在阐明miR-107影响肝缺血再灌注损伤（IRI）的分子机制。方法：采用实时荧光定量PCR技术，定量检测miR-107在肝脏IRI中的表达变化。随后，通过CCK-8、流式细胞仪和商用试剂盒进行体外细胞实验，验证miR-107对缺氧/再氧化（HR）的作用。机制方面，通过荧光素酶报告基因实验确定miR-107与靶基因存在调控关系。结果：小鼠肝脏IRI中，miR-107表达升高，HMOX1表达降低。在体外细胞实验中，miR-107抑制剂有利于减轻hr损伤的肝窦内皮细胞的增殖、凋亡、炎症和氧化应激。在分子机制研究中，我们确定miR-107可以结合HMOX1并抑制HMOX1的表达。低HMOX1表达可消除miR-107抑制剂的保护作用。结论：MiR-107在肝脏IRI中表达升高，并通过靶向HMOX1抑制加重肝损伤。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Hereditas Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.80

自引率

3.70%

发文量

期刊介绍： For almost a century, Hereditas has published original cutting-edge research and reviews. As the Official journal of the Mendelian Society of Lund, the journal welcomes research from across all areas of genetics and genomics. Topics of interest include human and medical genetics, animal and plant genetics, microbial genetics, agriculture and bioinformatics.