Alima Khanam, Sadnima Zaman, Md Aminul Islam, Suzzada Suhi, Md Rezoan Hossain, Mohammad Shariar Shovon, Takumi Saito, Takahiro Hirano, Yasuhiro Ozeki, Yuki Fujii, A K M Asaduzzaman, Syed Rashel Kabir, Imtiaj Hasan
{"title":"Novel mannose-binding snail mucus lectins from Lissachatina fulica exhibited antimicrobial, antitumor and insecticidal activities.","authors":"Alima Khanam, Sadnima Zaman, Md Aminul Islam, Suzzada Suhi, Md Rezoan Hossain, Mohammad Shariar Shovon, Takumi Saito, Takahiro Hirano, Yasuhiro Ozeki, Yuki Fujii, A K M Asaduzzaman, Syed Rashel Kabir, Imtiaj Hasan","doi":"10.1016/j.fsi.2025.110565","DOIUrl":null,"url":null,"abstract":"<p><p>Lectins are carbohydrate-binding proteins with diverse biological functions, particularly their roles in innate immunity through the recognition of pathogen-associated molecular patterns and mediation of non-specific immune responses. This study investigates the bioactivities of mucus lectins derived from Lissachatina fulica (LFLs). The LFLs were purified using ion exchange chromatography and analyzed for toxicity, antimicrobial, antioxidant, antitumor, and insecticidal properties. SDS-PAGE experiment revealed protein bands of approximately 70.0 ± 1.0 and 49.0 ± 1.0 kDa. Hemagglutination activity of LFLs was strongly inhibited by D-mannose, whereas galactose and N-acetyl-D-glucosamine showed moderate and mild inhibition, respectively. LFLs exhibited full activity between 20 and 50°C and at pH 7-9. Significant toxicity was observed in the brine shrimp lethality assay (LC<sub>50</sub> = 172.3 μg/ml). Antimicrobial assays showed dose-dependent inhibition against Shigella boydii, Shigella sonnei, Bacillus cereus, and Staphylococcus aureus within a concentration range of 50-400 μg/ml. LFLs also exhibited antibiofilm properties targeting Escherichia coli and antifungal efficacy against Aspergillus niger. The DPPH scavenging assay showed moderate antioxidant potential (IC<sub>50</sub> = 696.09 μg/ml). In vivo, LFLs inhibited Ehrlich ascites carcinoma (EAC) cell growth by 31.14 % and 55.71 % at doses of 3.0 and 6.0 mg/kg/day, respectively, and improved both mean survival time and hematological parameters. LFLs demonstrated time-dependent larvicidal activity against Culex quinquefasciatus (LC<sub>50</sub> reduced from 4.17 to 0.23 mg/ml over 48 h) and insecticidal activity against Callosobruchus chinensis (LD<sub>50</sub> reduced from 0.218 % to 0.062 % over 48 h), indicating increased potency with longer exposure. These findings highlight the significant therapeutic and biopesticidal potential of LFLs, supporting further research into their applications.</p>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":" ","pages":"110565"},"PeriodicalIF":3.9000,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Fish & shellfish immunology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1016/j.fsi.2025.110565","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/7/14 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"FISHERIES","Score":null,"Total":0}
引用次数: 0
Abstract
Lectins are carbohydrate-binding proteins with diverse biological functions, particularly their roles in innate immunity through the recognition of pathogen-associated molecular patterns and mediation of non-specific immune responses. This study investigates the bioactivities of mucus lectins derived from Lissachatina fulica (LFLs). The LFLs were purified using ion exchange chromatography and analyzed for toxicity, antimicrobial, antioxidant, antitumor, and insecticidal properties. SDS-PAGE experiment revealed protein bands of approximately 70.0 ± 1.0 and 49.0 ± 1.0 kDa. Hemagglutination activity of LFLs was strongly inhibited by D-mannose, whereas galactose and N-acetyl-D-glucosamine showed moderate and mild inhibition, respectively. LFLs exhibited full activity between 20 and 50°C and at pH 7-9. Significant toxicity was observed in the brine shrimp lethality assay (LC50 = 172.3 μg/ml). Antimicrobial assays showed dose-dependent inhibition against Shigella boydii, Shigella sonnei, Bacillus cereus, and Staphylococcus aureus within a concentration range of 50-400 μg/ml. LFLs also exhibited antibiofilm properties targeting Escherichia coli and antifungal efficacy against Aspergillus niger. The DPPH scavenging assay showed moderate antioxidant potential (IC50 = 696.09 μg/ml). In vivo, LFLs inhibited Ehrlich ascites carcinoma (EAC) cell growth by 31.14 % and 55.71 % at doses of 3.0 and 6.0 mg/kg/day, respectively, and improved both mean survival time and hematological parameters. LFLs demonstrated time-dependent larvicidal activity against Culex quinquefasciatus (LC50 reduced from 4.17 to 0.23 mg/ml over 48 h) and insecticidal activity against Callosobruchus chinensis (LD50 reduced from 0.218 % to 0.062 % over 48 h), indicating increased potency with longer exposure. These findings highlight the significant therapeutic and biopesticidal potential of LFLs, supporting further research into their applications.
期刊介绍：
Fish and Shellfish Immunology rapidly publishes high-quality, peer-refereed contributions in the expanding fields of fish and shellfish immunology. It presents studies on the basic mechanisms of both the specific and non-specific defense systems, the cells, tissues, and humoral factors involved, their dependence on environmental and intrinsic factors, response to pathogens, response to vaccination, and applied studies on the development of specific vaccines for use in the aquaculture industry.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
